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  • [Determination of the relative RNA-content of liver cell nuclei with gallocyanin-chromealum-staining (author's transl)].

[Determination of the relative RNA-content of liver cell nuclei with gallocyanin-chromealum-staining (author's transl)].

Acta histochemica (1981-01-01)
K Wenzelides, G Korek, K Voss
ABSTRACT

The relative RNA-content of liver cell nuclei from histological slides of rat liver was determined by automatical microscope picture analysis. The slides were stained with Gallocyanin-Chromealum. Extinction measurements are made before and after perchloracid extraction of RNA for the same slide. The changes of extinction measurements caused by the changes of slide thickness and of tissue inhomogeneity could be lowered using this method.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Gallocyanine, Dye content 90 %
Sigma-Aldrich
Chromium(III) potassium sulfate dodecahydrate, ACS reagent, ≥98%
Sigma-Aldrich
Chromium(III) potassium sulfate dodecahydrate, purum p.a., ≥98.5% (RT)