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Merck

STBMTSB12DP

Tryptic Soy Broth Ready-to-use Bottles

bottle capacity 125 mL, bottle filling volume 100 mL, closure type, green screw cap with septum, double packed of 12 bottles

Synonym(s):

Caso bouillon, Soybean Casein Digest Broth, Soybean casein digest medium, TSB, Trypcase soya broth, Tryptic soy broth, Tryptone soya broth

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About This Item

UNSPSC Code:
41106225
eCl@ss:
32014001
NACRES:
NA.74

Key Documents

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Agency

in accordance with EP 2.6.1
in accordance with JP 4.06
in accordance with USP 71

Quality Level

400

sterility

sterile; ethylene oxide treated

form

liquid

feature

closure type green screw cap with septum
ready-to-use

packaging

double packed of 12 bottles

bottle capacity

125 mL

bottle filling volume

100 mL

input

sample type pharmaceutical(s)

pH

7.3

application(s)

pharmaceutical
sterility testing

compatibility

for use with Steritest® Symbio FLEX Pump Kit, 2 media (SYMBFLE01)
for use with Steritest® Symbio ISL Pump Kit, 2 media (SYMBISL01)
for use with Steritest® Symbio LFH Pump Kit (SYMBLFH01)
for use with Steritest®

shipped in

ambient

storage temp.

2-30°C

suitability

nonselective for

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General description

Soybean Casein Digest Broth is a sterility medium and a critical component of our STERITEST® solution. Composition and growth performance of this medium comply with the European Pharmacopeia (EP) 2.6.1, United States Pharmacopeia (USP) 71 and Japanese Pharmacopeia (JP) 4.06, for Soybean-Casein Digest Medium. Our manufacturing approach ensures the highest level of quality for our media, improving accuracy and significantly reducing the risk of incorrect interpretation and false results. The sterilized double Tyvek® packaging increases the test method reliability, allowing to streamline the cleaning procedures, minimize the risk of cross-contamination in laminar flow hoods and secure an efficient decontamination of isolator chambers. The appearance of the media is a light-yellow clear liquid. It has a pH of 7.3 ± 0.2 and the storage temperature is 2-25°C. The incubation temperature varies between 20-25°C, with an incubation time of 7-14 days. The product is supplied as 12 bottles of 100 ml of medium, screw cap with septum closure that minimizes the risks of cross-contamination and optimizes the disinfection procedures.

Application

Soybean-Casein Digest Medium is intended for the detection of aerobic bacteria and fungi. The QC organisms include Bacillus subtilis (ATCC 6633), Candida albicans (ATCC 10231), Aspergillus brasiliensis (niger) (ATCC 16404), Staphylococcus aureus (ATCC 6538) and Pseudomonas aeruginosa (ATCC 9027). This medium is used for sterility testing by membrane filtration or direct inoculation. It is also used as a pre-enrichment broth for nonsterile products.
Sterility testing (membrane filtration and direct inoculation)
Bioburden testing

Preparation Note

Trypticase Soy

Other Notes

Screw cap with septum

Legal Information

STERITEST is a registered trademark of Merck KGaA, Darmstadt, Germany
Tyvek is a registered trademark of E. I. du Pont de Nemours and Company

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Articles

Sterility Testing by Direct Inoculation

Sterility testing media options facilitate direct inoculation for non-filterable samples, including soybean casein digest and Fluid Thioglycollate medium.

Questions

  1. Is Specific gravity of TSB is mentioned in any of the documents?

    1 answer

    1. We do not test these specification. Theoretically the density of prepared TSB is 1.03 g/ml.

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