In catalog number FLAAS, the specification for our vial Content is 0.90 - 1.10 mg ATP/vial by Enzymatic Assay. Please refer to the specific lot certificate of analysis for an individual quantity.
A2383
Adenosine 5′-triphosphate disodium salt hydrate
≥99%, microbial, powder
Adenosine 5′-triphosphate disodium salt hydrate
Synonym(s):
ATP disodium salt
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1 G
RM 450.00
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RM 1,050.00
10 G
RM 1,613.00
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RM 3,952.00
RM 450.00
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About This Item
Empirical Formula (Hill Notation):
C10H14N5Na2O13P3 · xH2O
CAS Number:
Molecular Weight:
551.14 (anhydrous basis)
EC Number:
MDL number:
UNSPSC Code:
41106305
eCl@ss:
32160414
PubChem Substance ID:
NACRES:
NA.51
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Product Name
Adenosine 5′-triphosphate disodium salt hydrate, Grade I, ≥99%, from microbial
biological source
microbial
Quality Level
type
Grade I
Assay
≥99%
form
powder
solubility
H2O: 50 mg/mL
storage temp.
−20°C
SMILES string
O.[Na+].[Na+].Nc1ncnc2n(cnc12)[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)[C@@H](O)[C@H]3O
InChI
1S/C10H16N5O13P3.2Na.H2O/c11-8-5-9(13-2-12-8)15(3-14-5)10-7(17)6(16)4(26-10)1-25-30(21,22)28-31(23,24)27-29(18,19)20;;;/h2-4,6-7,10,16-17H,1H2,(H,21,22)(H,23,24)(H2,11,12,13)(H2,18,19,20);;;1H2/q;2*+1;/p-2/t4-,6-,7-,10-;;;/m1.../s1
InChI key
NTBQNWBHIXNPRU-MSQVLRTGSA-L
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This Item | A6419 | FLAAS | A3377 |
|---|---|---|---|
| biological source microbial | biological source microbial | biological source bacterial (Corynebacterium sp) | biological source microbial |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| assay ≥99% | assay ≥99% (HPLC) | assay ≥90% | assay ≥97% (HPLC) |
| form powder | form powder | form lyophilized powder | form crystalline |
| solubility H2O: 50 mg/mL | solubility H2O: 50 mg/mL | solubility - | solubility H2O: 50 mg/mL |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
Application
Adenosine 5′-triphosphate disodium salt hydrate has been used:
- to prepare adenosine triphosphate (ATP) standard solutions to determine ATP levels in various bacterial cultures[1]
- bone marrow-derived macrophages (BMDM) treatment[2]
- as a preparation free of contaminating guanine nucleotides to study the binding effects of coatomer β subunit (β-COP)[3]
Biochem/physiol Actions
Adenosine 5′-triphosphate (ATP) is a central component of energy storage and metabolism in vivo.[4] ATP is used in many cellular processes, respiration, biosynthetic reactions, active transport, and motility.[5] ATP is a substrate of many kinases involved in cell signaling and of adenylate cyclase that produce the secondary messenger cyclic adenosine monophosphate (cAMP).[6] ATP provides the metabolic energy to drive metabolic pumps.[5] It serves as a coenzyme in a wide array of enzymatic reactions.[7]
P2 purinergic agonist; increases activity of Ca2+-activated K+ channels; substrate for ATP-dependent enzyme systems
P2 purinocepter agonist.
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Rui Nian et al.
Biotechnology progress, 25(4), 1078-1085 (2009-06-25)
The use of polyethylene glycol (PEG) as a refolding additive to a refolding cocktail comprising the molecular bichaperone ClpB and DnaKJE significantly enhances chaperone-mediated refolding of heat-denatured malate dehydrogenase (MDH). The critical factor to affect the refolding yield is the
Release of extracellular ATP by bacteria during growth
Mempin R, et al.
BMC Microbiology, 13(1), 301-301 (2013)
J G Donaldson et al.
Proceedings of the National Academy of Sciences of the United States of America, 89(14), 6408-6412 (1992-07-15)
The coatomer is a cytosolic protein complex that reversibly associates with Golgi membranes and is implicated in modulating Golgi membrane transport. The association of beta-COP, a component of coatomer, with Golgi membranes is enhanced by guanosine 5'-[gamma-thio]triphosphate (GTP[gamma S]), a
Mass spectrometry and NMR spectroscopy based quantitative metabolomics
Proteomics. Clinical Applications (2020)
Physiology, Adenosine Triphosphate (ATP)
NCBI Bookshelf null
Protocols
Enzymatic assay of lipase type XIII from Pseudomonas sp. using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC 3.1.1.3)
Measure hexokinase activity using a continuous spectrophotometric rate-determination assay at 340 nm, catalyzing D-hexose sugar phosphorylation using ATP.
Measure luciferase activity using a luminometer assay detecting light emission, with applications in ATP detection and genetic function reporting.
Related Content
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I would like to know how many g of ATP disodium salt hydrate contains in FLAAS -1vol vial? Thanks
1 answer-
Helpful?
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What volume of water should 1mg ATP be reconstituted in & what would be its corresponding concentration?
1 answer-
This product is soluble in water up to 50 mg/mL. The appropriate stock concentration for assays can be determined by the end user. The molecular weight is 551.14 (anhydrous basis), and the water content can be found on the lot-specific certificate of analysis. Using the molecular weight, the water content, and the chosen concentration for the ATP solution, the molarity can be calculated.
Helpful?
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Can I dissolve this ATP in water and store it?
1 answer-
This item can be solubilized in water at 50 mg/mL yielding a clear, colorless solution. Aqueous solutions of ATP are stable for months at -15C and or one week when stored at -0C as mentioned in the Product Information Sheet.
For more information, please navigate to the ‘DOCUMENTATION’ section to access the Product Information Sheet under 'More Documents':
https://www.sigmaaldrich.com/product/sigma/a2383#product-documentationHelpful?
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