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Merck

F3040

Monoclonal ANTI-FLAG® M1 antibody

clone M1, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-ddddk, Anti-dykddddk

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F3040-.2MG

RM 3,325.00

F3040-1MG

RM 5,706.00

F3040-5MG

RM 10,479.00

RM 3,325.00

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About This Item

MDL number:
MFCD00212941
UNSPSC Code:
12352203
NACRES:
NA.32

Key Documents

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biological source

(Bioreactor)

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

M1, monoclonal

form

buffered aqueous solution

purified by

using Protein A

species reactivity

all

concentration

2-5 mg/mL

technique(s)

western blot: 10 μg/mL

immunogen sequence

DYKDDDDK

shipped in

dry ice

storage temp.

−20°C

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Monoclonal ANTI-FLAG® M1 antibody clone M1, purified immunoglobulin, buffered aqueous solution

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Monoclonal ANTI-FLAG® M1 antibody

Monoclonal ANTI-FLAG® M5 antibody produced in mouse clone M5, purified immunoglobulin, buffered aqueous solution

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F4042

Monoclonal ANTI-FLAG® M5 antibody produced in mouse

ANTI-FLAG® BioM2 antibody, Mouse monoclonal clone M2, purified immunoglobulin, buffered aqueous glycerol solution

Sigma-Aldrich

F9291

ANTI-FLAG® BioM2 antibody, Mouse monoclonal

ANTI-FLAG® M2 antibody, Mouse monoclonal 1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

Sigma-Aldrich

F1804

ANTI-FLAG® M2 antibody, Mouse monoclonal

conjugate

unconjugated

conjugate

unconjugated

conjugate

biotin conjugate

conjugate

unconjugated

species reactivity

all

species reactivity

all

species reactivity

all

species reactivity

all

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

biological source

(Bioreactor)

biological source

mouse

biological source

mouse

biological source

mouse

clone

M1, monoclonal

clone

M5, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

General description

The ANTI-FLAG M1 IgG2b mooclonal antibody binds to proteins with a FLAG marker at the free N-terminus.

Method of purification - Protein A

Immunogen

FLAG; peptide sequence DYKDDDDK

Application

Monoclonal ANTI-FLAG® M1 antibody is suitable for immunoprecipitation, western blotting, or EIA in yeast, animal and E.coli cells.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.


Chromatin immunoprecipitation (1 paper)


Immunofluorescence (1 paper)

Learn more product details in our FLAG® applications portal.

Biochem/physiol Actions

Binds to the FLAG epitope when it is located at the free amino-terminus of a fusion protein. Does not bind to Met-FLAG fusion proteins, so will not recognize unprocessed, cytoplasmically expressed proteins. Binding is Ca2+-dependent; the complex dissociates in the absence of calcium ions.

Physical form

Solution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v).

Preparation Note

Dilute antibody to 10ug/mL in .05 M TBS, pH 7.4, containing 1 mM CaCl2.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000466452
0000466452
0000410739
0000310613
0000310613

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Mohammad Al Sorkhy et al.
BMC cancer, 12, 45-45 (2012-01-28)
Spy1 is a novel 'cyclin-like' activator of the G1/S transition capable of enhancing cell proliferation as well as inhibiting apoptosis. Spy1 protein levels are tightly regulated during normal mammary development and forced overexpression in mammary mouse models accelerates mammary tumorigenesis.
Reem Berro et al.
Journal of virology, 82(14), 7155-7166 (2008-05-16)
The human immunodeficiency virus type 1 (HIV-1) Tat is a 14-kDa viral protein that acts as a potent transactivator by binding to the transactivation-responsive region, a structured RNA element located at the 5' end of all HIV-1 transcripts. Tat transactivates
Andrew Best et al.
Nature communications, 5, 4760-4760 (2014-09-12)
Alternative splicing--the production of multiple messenger RNA isoforms from a single gene--is regulated in part by RNA binding proteins. While the RBPs transformer2 alpha (Tra2α) and Tra2β have both been implicated in the regulation of alternative splicing, their relative contributions
Min Luo et al.
Cell research, 20(2), 211-222 (2010-01-27)
As a critical apoptosis executioner, caspase-3 becomes activated and then enters into the nucleus to exert its function. However, the molecular mechanism of this nuclear entry of active caspase-3 is still unknown. In this study, we revealed that caspase-3 harbors
Chandrahasa R Yellaturu et al.
The Journal of biological chemistry, 284(46), 31726-31734 (2009-09-18)
The regulation of lipid homeostasis by insulin is mediated in part by the enhanced transcription of the gene encoding sterol regulatory element-binding protein-1c (SREBP-1c). The nascent SREBP-1c is embedded in the endoplasmic reticulum (ER) and must be transported to the
View All Related Papers

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