This product is provided as a clear, viscous solution. Refrigeration may cause it to become difficult to use for coverslipping. Its product Information states, "Do not refrigerate". This applies to storage after the product has been opened.
F4680
Fluoromount™ Aqueous Mounting Medium
liquid
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Product Name
Fluoromount™ Aqueous Mounting Medium, for use with fluorescent dye-stained tissues
Quality Level
form
liquid
technique(s)
microbe id | staining: suitable
color
clear
suitability
suitable for fluorescence
suitable for immunohistochemistry
application(s)
diagnostic assay manufacturing
hematology
histology
1 of 4
This Item | F6057 | SRA1 | M1942 |
|---|---|---|---|
| suitability suitable for immunohistochemistry | suitability - | suitability - | suitability - |
| grade for fluorescence | grade - | grade - | grade - |
| technique(s) microbe id | staining: suitable | technique(s) immunofluorescence: suitable | technique(s) - | technique(s) thin layer chromatography (TLC): suitable |
| color clear | color - | color - | color - |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| form liquid | form liquid | form liquid | form liquid |
General description
Fluoromount is an aqueous based mounting medium,similar to Gel Mountä, for use with coverslips to preserve tissues stained with fluorescent dyes. Thisproduct is compatible with FITC, phycoerythrin, phycocyanin, and allophycocyanin labeling. It can alsobe used with other fluorescent markers such as TexasRedÒand rhodamine. The medium after use can easily be removed by soaking the slides in deionized water.
Application
Legal Information
Fluoromount is a trademark of Diagnostic BioSystems, Inc.
related product
Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Instructions
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Can you explain why it's recommended not to refrigerate it? Does that still apply even after opening?
1 answer-
Helpful?
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What are some examples of aqueous mounting media without glycerol?
1 answer-
For use with fluorescence:
1. F4680 Fluoromount
2. F6182 FluoroshieldAqueous Mounting media with no glycerol and antifade agents:
1. 10981 Polyvinyl alcohol with DABCO
2. F6937 Fluoroshield with DABCO
3. F6812 Fluoroshield with PPD (not suitable for a select number of fluorescent dyes)Aqueous Mounting Media with no glycerol and another dye:
1. F6057 Fluoroshield with DAPI
2. F5932 Fluoroshield with PIAqueous Mounting Media suitable for use with light microscopy:
1.GG1 Glycerol gelatin
2.C9368 CC/MountHelpful?
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Is F4680 suitable for use as a mounting medium for AEC red IHC mounting?
1 answer-
Fluoromount is not intended for use with bright field microscopy or for coverslipping slides stained with AEC. Most fluorescent mounting media are somewhat alkaline, with a pH around 9, while AEC is typically formulated with an acetate buffer around pH 5.2. The difference in pH levels suggests that the AEC may fade quickly, especially if the slides are counterstained with a nuclear counterstain such as Mayer's Hematoxylin, which would also likely disappear rapidly.
Helpful?
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Does this (Fluoromount F4680) contain DAPI?
1 answer-
This product does not contain DAPI. Should DAPI be preferred, see product F6057.
F4680 Fluoromount datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/692/371/f4680pis.pdfF6057 Fluoroshield with DAPI product page:
https://www.sigmaaldrich.com/product/sigma/f6057Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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Does Fluoromount mounting media (Product No. F4680) contain glycerol?
1 answer-
Fluoromount does not contain glycerol. The exact formulation is considered proprietary.
Helpful?
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How does Product F4680, Fluoromount Mounting Medium, compare to GelMount (Product No. G0918) which has been deleted?
1 answer-
Product No. F4608, Fluoromount is essential the same as GelMount. GelMount was deleted by the supplier. Fluoromount is being made by another supplier with the same formulation.
Helpful?
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Does Fluoromount mounting media (Product No. F4680) contain an antifade reagent?
1 answer-
Fluoromount does not contain an antifade reagent. If you need an antifade mounting media, check Fluoroshield (Product Nos. F6182, F6057 and F5932) or PVA with antifade (Product Nos. 10981 and 10979).
Helpful?
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How do you remove the coverslip after using Fluoromount (Product No. F4680)?
1 answer-
Place the slide in a beaker full of deionized water on a magnetic stirrer. Gently stir the slide for a few hours to overnight for complete removal of dry Fluoromount.
Helpful?
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Does Fluoromount (Product No. F4680) remain a liquid or solidify?
1 answer-
Fluoromount will solidify as it dries. This typically takes 30-40 minutes.
Helpful?
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