H7523

HEPES

BioXtra, pH 5.0-6.5 (1 M in H₂O), ≥99.5% (titration)

• Synonym(s): 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
• Empirical Formula (Hill Notation): C₈H₁₈N₂O₄S
• CAS Number: 7365-45-9
• Molecular Weight: 238.30
• UNSPSC Code: 12161700
• NACRES: NA.25
• PubChem Substance ID: 24895796
• EC Number: 230-907-9
• Beilstein/REAXYS Number: 883043
• MDL number: MFCD00006158

Properties

• Product Name: HEPES, BioXtra, pH 5.0-6.5 (1 M in H₂O), ≥99.5% (titration)
• InChI key: JKMHFZQWWAIEOD-UHFFFAOYSA-N
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• SMILES string: OCCN1CCN(CC1)CCS(O)(=O)=O
• product line: BioXtra
• assay: ≥99.5% (titration)
• form: powder
• impurities: Insoluble matter, passes filter test
• ign. residue (900°C): ≤0.1% (as SO₄)
• loss: ≤1.0% loss on drying, 110°C
• pH: 5.0-6.5 (1 M in H₂O)
• useful pH range: 6.8-8.2
• pKa (25 °C): 7.5
• solubility: H₂O: 1 M, clear, colorless
• anion traces: chloride (Cl^-): ≤0.05%
• cation traces: Al: ≤0.0005%; As: ≤0.0001%; Ba: ≤0.0005%; Bi: ≤0.0005%; Ca: ≤0.001%; Cd: ≤0.0005%; Co: ≤0.0005%; Cr: ≤0.0005%; Cu: ≤0.0005%; Fe: ≤0.0005%; K: ≤0.005%; Li: ≤0.0005%; Mg: ≤0.0005%; Mn: ≤0.0005%; Mo: ≤0.0005%; Na: ≤0.005%; Ni: ≤0.0005%; Pb: ≤0.0005%; Sr: ≤0.0005%; Zn: ≤0.0005%
• absorption: ≤0.035 at 280 in H₂O at 1 M; ≤0.045 at 260 in H₂O at 1 M
• application(s): diagnostic assay manufacturing
• Quality Level: 400

Description

Application

HEPES has been used:

• As a component of homogenisation buffer used in protein sample preparation[1]
• To neutralise PRP (platelet-rich plasma) solution as part of PRP preparation[2]
• As a component in 2X PBS buffer, which is used in phosphate calcium transfection in cell line preparation[3]
• As a supplement in RPMI 1640 medium used to culture human umbilical vein endothelial cells (HUVEC) and in M199 complete medium for ex-vivo treatment of testicular tissue.[4][5]

General description

HEPES has been described as one of the best all-purpose buffers available for biological research.[6] At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.[7]

Other Notes

Easily compare specifications for HEPES products with the HEPES specification table.

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)