L9031

Lipase from Mucor miehei

lyophilized powder, ≥4,000 units/mg solid (using olive oil)

• Synonym(s): Triacylglycerol acylhydrolase, Triacylglycerol lipase
• CAS Number: 9001-62-1
• EC Number: 3.1.1.3 (BRENDA, IUBMB)
• EC Number: 232-619-9
• MDL number: MFCD00131509
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• form: lyophilized powder
• Quality Level: 100
• specific activity: ≥4,000 units/mg solid (using olive oil)
• storage temp.: 2-8°C
• InChI: 1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
• InChI key: QWZUIMCIEOCSJF-CHHCPSLASA-N

Description

General description

Lipase is produced by several animals, microbes, and plants. Lipase from Mucor sp. is a fungal lipase.[1] It is a member of the serine hydrolase family.[2]

Application

Lipase from Mucor miehei can be used to drive the synthesis of flavor esters. [3]

Lipase from Mucor miehei has been used in a study to assess the nonthermal effect of microwave irradiation in nonaqueous enzymatic esterification. [4] It has also been used in a study to investigate the hydrolysis of virgin coconut oil using immobilized lipase in a batch reactor. [5]

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Biochem/physiol Actions

Mucor miehei lipase hydrolizes triglycerides and catalyzes the synthesis of esters from glycerol and long-chain fatty acids in vitro. Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

Microbial lipase has a wide industrial application due to its stability, broad substrate specificity, and selectivity.[1] It may be used in the synthesis of biosurfactants and finds application in organic chemical processing, dairy industry, oleochemical industry, detergent formulations, paper, cosmetics, nutrition, and the pharmaceutical industry.[6]

Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

Analysis Note

Protein determined by biuret.

Other Notes

One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.7 at 37 °C using olive oil.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves