P1415

Prehybridization Solution

for Northern and Southern blotting, liquid (2x concentrate)

• NACRES: NA.25
• UNSPSC Code: 12161700

Properties

• Quality Segment: 100
• form: liquid (2x concentrate)
• technique(s): nucleic acid detection: suitable
• foreign activity: DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
• storage temp.: -10 to -25°C

Description

General description

Sigma′s Prehybridization Solution is a 2x ready-to-use buffer for Northern and Southern blot protocols.

Application

Suitable for use as a pre-hybridization buffer in Northern and Southern blot protocols.

Preparation Note

Sigma′s 2X Prehybridization Solution contains 200 μg/mL DNA in 10x SSC and 2X Denhardt′s solutions:

• 200 μg/mL DNA
• 2mg Bovine Serum Albumin (BSA)
• 2mg Ficoll
• 2mg Polyvinylpyrrolidone (PVP)
• 0.15 M sodium citrate
• 1.5M NaCl

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable