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For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
T4661
Trizma® base
≥99.9% (titration), crystalline
Synonym(s):
2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
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RM 408.00
RM 1,813.00
RM 8,325.00
About This Item
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Product Name
Trizma® base, ≥99.9% (titration), crystalline
InChI key
LENZDBCJOHFCAS-UHFFFAOYSA-N
InChI
1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
SMILES string
NC(CO)(CO)CO
description
aminopeptidase substrate
assay
≥99.9% (titration)
form
crystalline
loss
≤5% (loss on drying, 110 °C)
color
white
pH
10.0-11.5
useful pH range
7-9
pKa (25 °C)
8.1
bp
219-220 °C/10 mmHg (lit.)
mp
167-172 °C (lit.)
solubility
water: soluble (400 g plus 600 mL of water)
λ
(290nm < 0.05)
in 5 mM Tris; DNA (40% (w/w))
application(s)
diagnostic assay manufacturing
Quality Level
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Related Categories
1 of 4
This Item | T1503 | T6791 | T6066 |
|---|---|---|---|
| assay ≥99.9% (titration) | assay ≥99.9% (titration) | assay ≥99.9% (titration) | assay ≥99.9% (titration) |
| Quality Level 400 | Quality Level 300 | Quality Level 400 | Quality Level 400 |
| form crystalline | form crystalline | form crystalline | form crystalline powder |
| application(s) diagnostic assay manufacturing | application(s) cell analysis | application(s) diagnostic assay manufacturing | application(s) clinical research |
| solubility water: soluble (400 g plus 600 mL of water) | solubility methanol: soluble 26 mg/mL at 25 °C, ethylene glycol: soluble 79.1 mg/mL at 25 °C, water: soluble (678 g/l at 20 °C) | solubility H2O: 1 M, colorless, water: 678 g/L at 20 °C | solubility water: soluble (666 mg/ml, yielding a clear colorless solution) |
| pH 10.0-11.5 | pH 10.5-12 | pH 10.5-12.0 (1 M in H2O) | pH 10.0-11.5 |
Analysis Note
Application
- As a component of lysis buffer for cell disruption[1][2]
- In the preparation of TBE solution for PAGE (polyacrylamide gel electrophoresis)[3][2]
- In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLC[4]
- In capillary electrochromatography and UV analysis of tocopherols and tocotrienols[5]
General description
Other Notes
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Packaging
Legal Information
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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Should it be stored at 4℃??
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This product may be stored at room temperature. For products with no specified storage temperature given on the label or product page, ambient storage may be assumed. Products that require controlled temperature storage, such as refrigeration or freezer conditions, storage temperatures are published on the product label, Certificate of Analysis, the Safety Data Sheet (SDS).
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