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Merck

V900477

HEPES

99.5%, crystalline powder, reagent grade, Vetec™

Synonym(s):

4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

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About This Item

Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
7365-45-9
Molecular Weight:
238.30
Beilstein:
883043
EC Number:
230-907-9
MDL number:
MFCD00006158
UNSPSC Code:
12161700
PubChem Substance ID:
329829878

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Product Name

HEPES, Vetec™, reagent grade, 99.5%

grade

reagent grade

product line

Vetec™

Assay

99.5%

form

crystalline powder

pH

5.0-6.5 (25 °C, 238 g/L)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

solubility

water: 500 mg/mL, clear, colorless

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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1 of 4

This Item
H23830H6147PHR1428
HEPES Vetec™, reagent grade, 99.5%

Sigma-Aldrich

V900477

HEPES

HEPES reagent grade, ≥99% (titration), crystalline

Sigma-Aldrich

H23830

HEPES

HEPES BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)

Sigma-Aldrich

H6147

HEPES

HEPES Pharmaceutical Secondary Standard; Certified Reference Material

Supelco

PHR1428

HEPES

assay

99.5%

assay

≥99% (titration)

assay

≥99.5% (titration)

assay

-

grade

reagent grade

grade

reagent grade

grade

-

grade

certified reference material, pharmaceutical secondary standard

product line

Vetec™

product line

-

product line

BioXtra

product line

-

form

crystalline powder

form

crystalline

form

powder

form

-

solubility

water: 500 mg/mL, clear, colorless

solubility

water: 1 M, clear, colorless

solubility

water: 500 mg/mL, clear, colorless

solubility

-

pH

5.0-6.5 (25 °C, 238 g/L)

pH

-

pH

5.0-6.5 (25 °C, 238 g/L)

pH

5.0-6.5 (25 °C, 238 g/L)

General description

HEPES, a zwitterionic buffer having a pH range of 6.8-8.2, is one among the 20 Good′s buffers. It′s commonly used in cell culture applications as it helps in maintaining the physiological pH.[1][2]

Legal Information

Vetec is a trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
WXBF7048V
WXBF6449V
WXBF5193V
WXBF5195V
WXBF3353V

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Acid-base buffering in organ preservation solutions as a function of temperature: new parameters for comparing buffer capacity and efficiency
Baicu SC and Taylor MJ
Cryobiology, 45(1), 33-48 (2002)
Evidence-based guidelines for controlling pH in mammalian live-cell culture systems
Michl J, et al.
Communications biology, 2(1), 1-12 (2019)
P Dey et al.
Oncogene, 33(33), 4213-4225 (2013-10-01)
Estrogen receptor β (ERβ) is emerging as a critical factor in understanding prostate cancer biology. Although reduced in prostate cancer above Gleason grade 3, ERβ is a potential drug target at the initial stage of the disease. In human prostate
Bérangère Pinan-Lucarré et al.
Nature, 511(7510), 466-470 (2014-06-05)
Because most neurons receive thousands of synaptic inputs, the neuronal membrane is a mosaic of specialized microdomains where neurotransmitter receptors cluster in register with the corresponding presynaptic neurotransmitter release sites. In many cases the coordinated differentiation of presynaptic and postsynaptic
Frédéric Perros et al.
Journal of the American College of Cardiology, 65(7), 668-680 (2015-02-14)
Endothelial cell (EC) dysfunction plays a central role in the pathogenesis of pulmonary arterial hypertension (PAH), promoting vasoconstriction, smooth muscle proliferation, and inflammation. This study sought to test the hypothesis that nebivolol, a β1-antagonist and β2,3-agonist, may improve PAH and
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