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  • The application of sodium deoxycholate and Sephacryl-200 for the delipidation and separation of high density lipoprotein.

The application of sodium deoxycholate and Sephacryl-200 for the delipidation and separation of high density lipoprotein.

Experientia (1982-04-15)
H Robern
ABSTRACT

A method to remove lipids from human plasma high-density lipoprotein (HDL) was developed. The procedure required column chromatography on Sephacryl-200 in the presence of the bile salt sodium deoxycholate. The lipid free protein obtained retained the immunological properties of the native HDL.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Sephacryl®, 500-HR, MW range 40-20,000 kDa (dextrans)