Metabolic profiling of Vitex agnus castus leaves, fruits and sprouts: analysis by LC/ESI/(QqQ)MS and (HR) LC/ESI/(Orbitrap)/MS n.

Metabolic profiling of Vitex agnus castus leaves, fruits and sprouts: analysis by LC/ESI/(QqQ)MS and (HR) LC/ESI/(Orbitrap)/MS n.

Journal of pharmaceutical and biomedical analysis (2014-10-12)

Angela Mari, Paola Montoro, Gilda D'Urso, Mario Macchia, Cosimo Pizza, Sonia Piacente

ABSTRACT

Food supplements based on Vitex agnus castus L. (Verbenaceae) fruits, also known as chasteberry, are routinely used by women against somatic and psychic premenstrual symptoms such as depression, sadness or irritability. With the aim of highlighting the differences in the chemical profiles of cultivated fruits and different parts of wild plants (fruits, leaves and sprouts) of V. agnus castus, a method concerning with the quali-quantitative study of the derived hydroalcoholic extracts was carried out by using high-performance liquid chromatography coupled to electrospray negative ionization Orbitrap multicollisional high resolution mass spectrometry (LC/ESI/(Orbitrap)MS(n)) and high-performance liquid chromatography coupled to electrospray negative ionization triple quadrupole tandem mass spectrometry (LC/ESI/(QqQ)MS) in multiple reaction monitoring (MRM) mode.

MATERIALS

Product Number

Brand

Product Description

1424109

USP

Methyl alcohol, United States Pharmacopeia (USP) Reference Standard

02870

Sigma-Aldrich

Ethanol, ACS reagent, prima fine spirit, without additive, F15 o¹

02882

Sigma-Aldrich

Ethanol, purum, absolute ethanol, denaturated with 2% 2-butanone, A15 MEK¹, ≥99.8% (based on denaturant-free substance)

02857

Sigma-Aldrich

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695076

Sigma-Aldrich

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E7023

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27001

Sigma-Aldrich

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459828

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493546

Sigma-Aldrich

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399388

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459844

Sigma-Aldrich

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32221

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Ethanol, puriss. p.a., absolute, ≥99.8% (GC)

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Sigma-Aldrich

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33015

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459836

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W248703

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40347

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06473

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Sigma-Aldrich

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Sigma-Aldrich

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PHR1316

Supelco

Residual Solvent - Chloroform, Pharmaceutical Secondary Standard; Certified Reference Material

632546

Supelco

Methanol solution, contains 0.10 % (v/v) formic acid, UHPLC, suitable for mass spectrometry (MS), ≥99.5%

C7559

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288306

Sigma-Aldrich

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48075

Supelco

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437581

Sigma-Aldrich

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439142

Supelco

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322415

Sigma-Aldrich

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82762

Supelco

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372978

Sigma-Aldrich

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