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  • Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Analytical chemistry (2015-03-17)
Evgeni Eltzov, Avital Cohen, Robert S Marks
ABSTRACT

Indoor air pollution became a recent concern found to be oftentimes worse than outdoor air quality. We developed a tool that is cheap and simple and enables continuous monitoring of air toxicity. It is a biosensor with both a nondisposable (monitor) and disposable (calcium alginate pads with immobilized bacteria) elements. Various parameters to enhance its signal have been tested (including the effect of the pad's orientation, it's exposure to either temperature or time with the air toxicant analyte, and various concentrations thereof). Lastly, the sensor has demonstrated its ability to sense the presence of chemicals in a real, indoor environment. This is the first step in the creation of a sensitive and simple operative tool that may be used in different indoor environments.

MATERIALS
Product Number
Brand
Product Description

Supelco
Methanol, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Diethylene glycol, Pharmaceutical Secondary Standard; Certified Reference Material
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Tetrahydrofuran, contains 250 ppm BHT as inhibitor, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99.9%
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Sodium chloride solution, 0.9% in water, BioXtra, suitable for cell culture
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Piperidine, ReagentPlus®, 99%
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Chloroform, ACS reagent, ≥99.8%, contains amylenes as stabilizer
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Acetone, natural, ≥97%
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Piperidine, ≥99.5%, purified by redistillation
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Sodium chloride-35Cl, 99 atom % 35Cl
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Chloroform, anhydrous, contains amylenes as stabilizer, ≥99%
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N,N-Dimethylformamide, anhydrous, 99.8%
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Tetrahydrofuran, anhydrous, ≥99.9%, inhibitor-free
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Chloroform, suitable for HPLC, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
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Acetone, suitable for HPLC, ≥99.9%
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Diethylene glycol, ReagentPlus®, 99%
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Diethyl ether, contains 1 ppm BHT as inhibitor, anhydrous, ≥99.7%
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Triphenyl phosphite, 97%
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Methanol, anhydrous, 99.8%
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Sodium chloride solution, 0.85%
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Formaldehyde-12C solution, 20% in H2O, 99.9 atom % 12C
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Chloroform, anhydrous, ≥99%, contains 0.5-1.0% ethanol as stabilizer
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Sodium chloride, random crystals, 99.9% trace metals basis
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Acetone, ≥99%, FCC, FG
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Diethyl ether, puriss. p.a., ACS reagent, ≥99.8% (GC)
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Formaldehyde solution, ACS reagent, 37 wt. % in H2O, contains 10-15% Methanol as stabilizer (to prevent polymerization)
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Diethylene glycol, puriss. p.a., ≥99.0% (GC), colorless
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Sodium chloride, tested according to Ph. Eur.
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Formaldehyde solution, Molecular Biology, BioReagent, ≥36.0% in H2O (T)
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(±)-Epichlorohydrin, analytical standard
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Sodium chloride solution, BioUltra, Molecular Biology, ~5 M in H2O