- Synthesis and characterization of phosphodiester stationary bonded phases for liquid chromatography.
Synthesis and characterization of phosphodiester stationary bonded phases for liquid chromatography.
Talanta (2015-06-17)
Szymon Bocian, Bogusław Buszewski
PMID26078125
ABSTRACT
The new type of stationary bonded phases for liquid chromatography with immobilized artificial membrane properties was synthesized. Based on the modification of diol-bonded silica gel the phosphodiester stationary phases were obtained. The structures of synthesized material were confirmed by different physico-chemical techniques such as elemental analysis, infrared spectroscopy (FTIR), (13)C CP/MAS NMR and in reversed phase and hydrophilic interaction liquid chromatography, where hydrophobic and polar compounds were sufficiently separated. To present the influence of phosphate group on the retention properties, obtained material were compared with Diol-Ester C12 stationary phase.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Phenol solution, Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, BioReagent, Molecular Biology
Sigma-Aldrich
Phenol solution, Saturated with 0.01 M citrate buffer, pH 4.3 ± 0.2, BioReagent, Molecular Biology
Sigma-Aldrich
Phenol, puriss., meets analytical specification of Ph. Eur., BP, USP, ≥99.5% (GC), crystalline (detached)
Sigma-Aldrich
Phenol, puriss., meets analytical specification of Ph. Eur., BP, USP, 99.5-100.5% (GC)
Supelco
Methanol solution, contains 0.10 % (v/v) formic acid, UHPLC, suitable for mass spectrometry (MS), ≥99.5%
Sigma-Aldrich
Acetonitrile solution, contains 0.1 % (v/v) trifluoroacetic acid, suitable for HPLC