A8311

Antifoam 204

aqueous emulsion for bacterial and mammalian systems

• Synonym(s): organic antifoam
• MDL number: MFCD00130523
• UNSPSC Code: 12161901
• NACRES: NA.85

Properties

• Quality Level: 200
• form: emulsion (aqueous)
• concentration: 100% (organic)
• density: 1.01 g/mL at 25 °C
• application(s): microbiology
• storage temp.: room temp
• suitability: (Mammalian (suspension)); bacteria (fermentation)

Description

General description

Antifoam 204 is an organic antifoam used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production.

Application

Suitable for preventing or minimizing foaming in microbial fermentation systems and mammalian suspension cultures.
Antifoam 204 has been used:

• in the expression and purification of PUL (PLAP, Ufd3p, and Lub1p) domain[1]
• in fed-batch fermentation[2]
• to prevent bubble formation due in aeration in Luria Bertani (LB) culture medium for bacterial growth[3]
• in the batch fermentation with mineral medium[4]

Preparation Note

For use in microbiological media Sigma recommends a starting concentration of between 0.005% and 0.01%.
The optimal amount of antifoam required for various applications will need to be determined. Antifoam 204 is soluble in methanol, ethanol, toluene, xylene, perchloroethylene, and cold water at temperatures below 15 °C. It is insoluble in warm water and ethylene glycol.

Other Notes

Antifoam 204 is a 100% active components and a mixture of non-silicone organic defoamers in a polyol dispersion.

Contains 100% active components and is a mixture of non-silicone organic defoamers in a polyol dispersion. Can be sterilized repeatedly.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves