Skip to Content
Merck
  • Acidogenic fermentation of Scenedesmus sp.-AMDD: Comparison of volatile fatty acids yields between mesophilic and thermophilic conditions.

Acidogenic fermentation of Scenedesmus sp.-AMDD: Comparison of volatile fatty acids yields between mesophilic and thermophilic conditions.

Bioresource technology (2015-11-10)
Marvin Gruhn, Jean-Claude Frigon, Serge R Guiot
ABSTRACT

This study compared the acidogenic fermentation of Scenedesmus sp.-AMDD at laboratory-scale, under mesophilic (35°C) and thermophilic conditions (55°C). Preliminary batch tests were performed to evaluate best conditions for volatile fatty acid (VFA) production from microalgal biomass, with respect to the inoculum, pH and nutrients. The use of bovine manure as inoculum, the operating pH of 4.5 and the addition of a nutrient mix, resulted in a high VFA production of up to 222mgg(-1) total volatile solid (TVS), with a butyrate share of 27%. Both digesters displayed similar hydrolytic activity with 0.38±0.02 and 0.42±0.03 g soluble chemical oxygen demand (COD)g(-1) TVS for the digesters operated at 35 and 55°C, respectively. Mesophilic conditions were more favorable for VFA production, which reached 171±5, compared to 88±12 mg soluble CODg(-1) TVS added under thermophilic conditions (94% more). It was shown that in both digesters, butyrate was the predominant VFA.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Methanol, suitable for NMR (reference standard)
Sigma-Aldrich
Methanol, anhydrous, 99.8%
Supelco
Ethanol solution, certified reference material, 2000 μg/mL in methanol
Sigma-Aldrich
1-Butanol, anhydrous, 99.8%
Sigma-Aldrich
1-Propanol, ≥99%, FG
Sigma-Aldrich
Sulfuric acid, 99.999%
Sigma-Aldrich
Isopropyl alcohol, ≥99.7%, FCC, FG
Supelco
Ethanol standards 10% (v/v), 10 % (v/v) in H2O, analytical standard
Sigma-Aldrich
Methanol, Absolute - Acetone free
Sigma-Aldrich
Sulfuric acid, puriss. p.a., for determination of Hg, ACS reagent, reag. ISO, reag. Ph. Eur., 95.0-97.0%
Sigma-Aldrich
Methanol, ACS reagent, ≥99.8%
Sigma-Aldrich
2-Butanol, puriss. p.a., reag. Ph. Eur., ≥99.5% (GC)
Sigma-Aldrich
Methanol, ACS spectrophotometric grade, ≥99.9%
Sigma-Aldrich
1-Butanol, ACS reagent, ≥99.4%
Sigma-Aldrich
Ethyl alcohol, Pure 190 proof, for molecular biology
Sigma-Aldrich
1-Propanol, ACS reagent, ≥99.5%
Sigma-Aldrich
1-Butanol, 99.9%
Sigma-Aldrich
Methanol, puriss., meets analytical specification of Ph Eur, ≥99.7% (GC)
Sigma-Aldrich
Methanol, BioReagent, ≥99.93%
Sigma-Aldrich
Ethyl alcohol, Pure, 200 proof, anhydrous, ≥99.5%
Sigma-Aldrich
Butyl alcohol, natural, ≥99.5%, FCC, FG
Sigma-Aldrich
Ethyl alcohol, Pure, 190 proof, meets USP testing specifications
Sigma-Aldrich
Methane-12C, 13C-depleted, 99.9 atom % 12C
Sigma-Aldrich
2-Propanol, BioUltra, Molecular Biology, ≥99.5% (GC)
Sigma-Aldrich
2-Propanol, anhydrous, 99.5%
Sigma-Aldrich
2-Propanol, BioReagent, ≥99.5%, Molecular Biology
Sigma-Aldrich
2-Propanol, electronic grade, 99.999% trace metals basis
Sigma-Aldrich
tert-Butanol, ≥99% (GC)
Sigma-Aldrich
Ethanol, purum, fine spirit, denaturated with 4.8% methanol, F25 METHYL1, ~96% (based on denaturant-free substance)
Sigma-Aldrich
Ethanol, purum, absolute ethanol, denaturated with 1% cyclohexane, A15 CYCLO1, ≥99.8% (based on denaturant-free substance)