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Urine Concentration with Amicon® Ultra Centrifugal Filters

Accurate measurement of specific proteins in urine is important for diagnosing and managing diseases.

Simple dialysis of Small Volume Samples using V Series Membranes

This article is a protocol for Simple dialysis of small volumes using V Series membranes

AliCE® Cell-Free Protein Synthesis Kit Quick Start Guide

Follow these five quick steps to perform cell-free protein synthesis using the ALiCE® protein expression plant lysate kit.

cOmplete™ Protocol

It is possible dissolve 1 tablet in as little as 2 ml double distilled Water, results in a 25x stock solution, without difficulty.

Duolink® In Situ Short Instructions - Fluorescence

This page details the Duolink® In Situ Short Protocol for fluorescence detection

Next Generation Cell Free Protein Expression Kit (Wheat Germ) (CFPS700) PROTOCOL

Protein synthesis using cell-free protein synthesis reagent kit consists of three stages: preparation of transcription template, transcription and translation.

Measuring Kinase Inhibitor Residence Times

Jump dilution protocol to determine the residence time of a drug (or drug candidate) during its interaction with a kinase using a fluorescence polarization assay based on the detection of ADP.

Yale Immunofluorescence Protocol

Procedure for Fixation, Immunostaining, and Imaging in 384-well Plates.

Yeast Transformation Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.

Calf Intestinal (CIP) Alkaline Phosphatase for Nucleic Acid Dephosphorylation

CIP is used to remove 5’-phosphate groups from DNA, RNA and both ribo and deoxy-ribonucleoside triphosphates. Detailed protocol on how to dephosphorylate DNA.

Alkaline Phosphatase Protocol

Alkaline phosphatase (AP) is a non-specific phosphomonoester hydrolase that catalyzes the hydrolysis of a wide variety of organic monophosphates.

Clean-up of Antibody Labeling Reactions Using Amicon Ultra Filters

Using a standard Amicon® Ultra centrifugal filter, a few rounds of diafiltration can efficiently remove unincorporated label from an antibody labeling reaction without diluting the sample.

Conjugating Alkaline Phosphatase to Antibodies and Other Proteins

Protocol for the one-step, glutaraldehyde coupling method to conjugation alkaline phosphatase to antibodies and other proteins.

Coupling Protocol for Primary Amine of a Ligand

This page shows coupling through the primary amine of a ligand with a NHS-activated Sepharose High Performance, NHS-activated Sepharose 4 Fast Flow and CNBr-activated Sepharose from Cytiva.

Enzymatic Deglycosylation

Enzymatic Deglycosylation Strategies: Sequential hydrolysis of individual monosaccharides from glycans can be useful for the elucidation of the structure and function of the glycan component.

Neuraminidase (Sialidase) Protocol

Neuraminidase can be used to cleave sialic acids from proteins. In this protocol, the enzyme from Vibrio cholerae is used on fixed cells.

Peptide Quick Tips

Peptide Quick Tips. Helpful guidelines for proper storage and resuspension of your peptide for optimal performance in your assay.

Purification or Removal of Biotin and Biotinylated Substances

This page shows how to purify or remove biotin and biotinylated substances with Streptavidin Sepharose High Performance from Cytiva.

Removal of GST Tag by Enzymatic Cleavage

This page how to remove GST tags by enzymatic cleavage using Cytiva products.

Tag Removal by Enzymatic Cleavage

This protocol shows how to remove histidine tags by enzymatic cleavage using Cytiva products.

Cell Disruption and Membrane Preparation

This page shows how to perform a cell disruption und prepare membranes with products from Cytiva.

cOmplete™, EDTA-free Protocol

It is possible dissolve 1 tablet in 2 mL double dist. water (results in a 25x stock solution) without any problems.

Processing Small Tissue or Cell Samples using Sample Grinding Kit

Sample Grinding Kit from Cytiva is designed to disrupt cell or tissue samples for protein extraction.

Detergent-Free Nuclear Protein Extraction

Detergent-free procedure is recommended for nuclear protein preparation to avoid interference with labeling efficiency of extracted proteins.

Handling Inclusion Bodies in Recombinant Protein Expression

How to isolate proteins from inclusion bodies using Cytiva products.

Liberase™ DH Research Grade Protocol & Troubleshooting

Your current tissue dissociation protocol, and the extent to which you have been successful with traditional collagenase are good guides for selecting the appropriate Liberase Enzyme Blend.

Liberase™ DL Research Grade Protocol & Troubleshooting

Your current tissue dissociation protocol, and the extent to which you have been successful with traditional collagenase are good guides for selecting the appropriate Liberase Enzyme Blend.

Liberase™ TH Protocol & Troubleshooting

Liberase Enzyme Blend selection depends on tissue dissociation protocol and success with collagenase.

Liberase™ TL Research Grade Protocol & Troubleshooting

Liberase™ TM Research Grade Protocol & Troubleshooting

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