PF033

Fas Ligand, Human, Recombinant

Fas Ligand, Human, Recombinant, consists of amino acids 103-281 fused at the N-terminus to a tag-linker peptide. Binds to human, rat, and mouse Fas.

• Synonym(s): CD178, CD95L, CD95L, CD178, NACP
• NACRES: NA.77
• UNSPSC Code: 12352202
• Form: lyophilized
• Assay: ≥95% (SDS-PAGE)
• Recombinant: expressed in HEK 293 cells

Properties

• recombinant: expressed in HEK 293 cells
• Quality Segment: 100
• assay: ≥95% (SDS-PAGE)
• form: lyophilized
• potency: 50 ng/mL ED₅₀ (A20 cells)
• does not contain: preservative
• species reactivity: human, mouse, rat
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• impurities: <0.1 EU/μg Endotoxin (EU/μg purified protein)
• solubility: soluble (Reconstitute in 50 μl H2O to yield a final stock concentration of 100 μg/ml. Further dilute in culture medium containing 5% FCS immediately prior to use.)
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Recombinant, human, Fas ligand consisting of amino acids 103-281 fused at the N-terminus to a tag-linker peptide. Binds to human, mouse, and rat Fas. The recombinant protein is produced in HEK293 cells. Useful for cytotoxicity assays.



Fas/APO1/CD95 (37-42 kDa), a member of the Tumor Necrosis Factor/Nerve Growth Factor (TNF/NGF) receptor family, is an apoptosis-signaling surface receptor known to trigger apoptotic cell death. Homology exists between the Fas and TNF receptors including a region called the "death domain" which is required to propagate the apoptotic signal. The signal activates a family of cysteine proteases, or caspases, which systematically lead to cell destruction. Genetic defects in the Fas system have been shown to lead to autoimmune disorders while increased activity or deregulation contributes to the pathology of diseases such as AIDS.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

Lyophilized from PBS.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Reconstitute in 50 μl H₂O to yield a final stock concentration of 100 µg/ml. Further dilute in culture medium containing 5% FCS immediately prior to use.

Other Notes

ED50:50 ng/mL (A20 cells).

Noguchi, K. et al. 1996. Oncogene13, 39.
Golstein, P. et al. 1995. Cell81, 185.
Katsikis, P. et al. 1995. J. Exp. Med.181, 2029.
Nagata, S. et al. 1995. Science267, 1449.
Thompson, C., 1995. Science267, 1456.
Watanabe-Fukunaga, R. et al. 1992. Nature356, 314.

The soluble Fas ligand kills APO-1/Fas-sensitive cells at a concentration of >50 ng/ml. For example, cell death results when 5 x 10⁵ murine A20 B lymphoma cells are incubated with 50-500 ng/ml of the recombinant human soluble APO1/FasL (aa 103-281) for 16 h at 37°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 1