Wszystkie zdjęcia(1)

07-052

Sigma-Aldrich

Anti-phospho-Phospholamban (Ser16) Antibody

Upstate®, from rabbit

Synonim(y):
cardiac phospholamban
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

rabbit

Poziom jakości

100

forma przeciwciała

affinity purified immunoglobulin

antibody product type

primary antibodies

klon

polyclonal

oczyszczone przez

affinity chromatography

species reactivity

mouse, human, canine, pig, rat, bovine, rabbit

manufacturer/tradename

Upstate®

zastosowania

western blot: suitable

izotyp

IgG

numer dostępu NCBI

numer dostępu UniProt

transport

dry ice

Opis ogólny

Phospholamban (PLB) is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1 to 25) contains the phosphorylation sites and is highly basic and readily cleaved by proteases; whereas the transmembrane domain (residues 25 to 52) is mostly hydrophobic, protease resistant and stabilizes the pentamer. Following adrenergic stimulation of cardiac muscle, PLB is phosphorylated at Ser16 and at Thr17 which is correlated with stimulation of calcium transport activity across the SR membrane and relaxation of cardiac fibers.

Specyficzność

Predicted to cross-react with human, mouse, rat, rabbit, pig and dog.
Recognizes phosphorylated phospho-lamban (Ser16), Mr 5-25 kDa. Additional non-specific bands may be detected, Mr 75 & 100 kDa, in some lysates.

Immunogen

KLH-conjugated, synthetic peptide corresponding to amino acids 14-25 of human cardiac phospholamban (RA[pS]TIEMPQQAR-C) with an added C-terminal cysteine. The immunizing sequence is identical in mouse, rat, rabbit, pig and dog.

Zastosowanie

Research Category
Metabolism

Signaling
This Anti-phospho-Phospholamban (Ser16) Antibody is validated for use in WB for the detection of phospho-Phospholamban (Ser16).
Research Sub Category
Muscle Physiology

General Post-translation Modification

Jakość

Routinely evaluated by western blot on bovine cardiac preparation that was incubated with PKA.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected phosphorylated phospholamban from a bovine cardiac preparation that was incubated with 10 µg PKA (Catalog # 14-114) for 10 minutes.
DO NOT BOIL CARDIAC PREPARATION.
Boiling may reduce the 25 kDa pentamer into tri-, di- or monomers. In addition, phosphorylation may disrupt the pentamer into dimers and monomers.

Opis wartości docelowych

Varies

Postać fizyczna

Purified rabbit polyclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol. Store at -20°C.
Immunoaffinity Chromatography

Przechowywanie i stabilność

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Komentarz do analizy

Control
Bovine cardiac preparation, treated with PKA.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informacje prawne

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Kod klasy składowania

10 - Combustible liquids

WGK

WGK 1

Certyfikat analizy

Świadectwo pochodzenia

Bhavini B Patel et al.
Toxicological sciences : an official journal of the Society of Toxicology, 133(1), 174-185 (2013-02-19)
Bisphenol A (BPA) is an estrogenizing endocrine disruptor compound of concern. Our objective was to test whether lifelong BPA would impact cardiac structure/function, calcium homeostasis protein expression, and the DNA methylation of cardiac genes. We delivered 0.5 and 5.0 µg/kg/day
Single histidine-substituted cardiac troponin I confers protection from age-related systolic and diastolic dysfunction.
Palpant, NJ; Day, SM; Herron, TJ; Converso, KL; Metzger, JM
Cardiovascular Research null
E J Mayer et al.
Biochemical and biophysical research communications, 267(1), 40-48 (2000-01-07)
Quantitative immunoassays to discriminate and quantitate phospholamban and its phosphorylation states in heart homogenates were developed using known amounts of protein determined by amino acid analysis. Synthetic 1-52 phospholamban, the hydrophilic 1-25 peptide, and 1-25 phosphopeptides containing P-Ser(16), P-Thr(17), and
Viviana Meraviglia et al.
International journal of molecular sciences, 19(2) (2018-02-01)
SERCA2a is the Ca2+ ATPase playing the major contribution in cardiomyocyte (CM) calcium removal. Its activity can be regulated by both modulatory proteins and several post-translational modifications. The aim of the present work was to investigate whether the function of
Li-Yen R Hu et al.
Science advances, 3(6), e1603081-e1603081 (2017-06-21)
Obscurins are cytoskeletal proteins with structural and regulatory roles encoded by OBSCN. Mutations in OBSCN are associated with the development of hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM). Specifically, the R4344Q mutation present in immunoglobulin domain 58 (Ig58) was the

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