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A7481

Sigma-Aldrich

Anti-Atg1/ULK1 antibody produced in rabbit

enhanced validation

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):
Anti-UNC51, Anti-Unc-51 like kinase 1 (C. elegans), Anti-Unc51.1
NACRES:
NA.41

pochodzenie biologiczne

rabbit

Poziom jakości

200

białko sprzężone

unconjugated

forma przeciwciała

affinity isolated antibody

antibody product type

primary antibodies

klon

polyclonal

forma

buffered aqueous solution

masa cząsteczkowa

antigen ~150 kDa

species reactivity

human (predicted), mouse, rat

rozszerzona walidacja

recombinant expression
Learn more about Antibody Enhanced Validation

stężenie

~1 mg/mL

technique(s)

western blot: 0.5-1.0 μg/mL using whole extracts of HEK-293T cells expressing mouse ULK1
western blot: 3-6 μg/mL using whole extracts of rat PC12

numer dostępu UniProt

transport

dry ice

temp. przechowywania

−20°C

Gene Information

human ... ULK1(8408)
mouse ... Ulk1(22241)
rat ... Ulk1(360827)

Opis ogólny

Atg1 is an autophagy protein that functions as a serine/threonine kinase in yeast to regulate autophagasome formation. Atg1 is ULK1 in mammals.
Unc-51 like autophagy activating kinase 1 (ULK1) gene is located on human chromosome 12q24.33.

Specyficzność

Anti- Atg1/ULK1 recognizes rat and mouse Atg1/ULK1.

Immunogen

Synthetic peptide corresponding to amino acids of mouse Atg1/ULK1 conjugated to KLH via a cysteine residue. The corresponding sequence is identical in rat and differs by 3 amino acids in human.

Zastosowanie

Anti-Atg1/ULK1 antibody produced in rabbit has been used in western blotting and immunohistochemical staining.

Działania biochem./fizjol.

Unc-51 like autophagy activating kinase 1 (ULK1) regulates autophagy initiation and innate immunity of interferons (IFNs). ULK1 is critical for neuronal development. It is essential for neurite elongation and differentiation of cerebellar granule neurons. Mutations in this gene is linked to latent mycobacterium tuberculosis infection (LTBI).

Postać fizyczna

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Przechowywanie i stabilność

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Kod klasy składowania

12 - Non Combustible Liquids

Temperatura zapłonu °F

Not applicable

Temperatura zapłonu °C

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificate of Analysis

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Certificate of Origin

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SDS

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