C2230

Coelenterazine, native

solid

• Synonim(y): 3,2-Dihydro-2-(p-hydroxybenzyl)-6-(p-hydroxyphenyl)-8-benzylimidazolo[1,2-a]pyrazin-3-one, CLZN
• Wzór empiryczny (zapis Hilla): C₂₆H₂₁N₃O₃
• Numer CAS: 55779-48-1
• Masa cząsteczkowa: 423.46
• NACRES: NA.32
• PubChem Substance ID: 24892477
• UNSPSC Code: 12352200
• MDL number: MFCD00467176

Właściwości

• assay: ≥95% (TLC)
• form: solid
• solubility: methanol and ethanol: soluble
• storage temp.: −20°C
• SMILES string: Oc1ccc(CC2=NC3=C(Cc4ccccc4)NC(=CN3C2=O)c5ccc(O)cc5)cc1
• InChI: 1S/C26H21N3O3/c30-20-10-6-18(7-11-20)15-23-26(32)29-16-24(19-8-12-21(31)13-9-19)27-22(25(29)28-23)14-17-4-2-1-3-5-17/h1-13,16,27,30-31H,14-15H2
• InChI key: YHIPILPTUVMWQT-UHFFFAOYSA-N

Opis

General description

Coelenterazine is a luciferin molecule, which is utilized by several luciferases, such as Renilla, Oplophorus and Periphylla.

Application

Coelenterazine, native was used:-

• For Aequorin assays
• For measuring the intracellular calcium concentration in response to ionomycin or PMA using aequorin in COS-7 cells
• In Bioluminescence imaging for analysing the presence of virus-dependent luciferase activity

Biochem/physiol Actions

Luminophore of the aequorin complex used to measure Ca²⁺ concentration.

Luminophore of the aequorin complex which is oxidized by oxygen to illuminate at 465 nm when Ca²⁺ binds to the complex; used to measure Ca²⁺ concentration in cells with high sensitivity and large dynamic range.

Disclaimer

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• Co się dzieje?: WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• PPE (środki ochrony indywidualnej): Eyeshields, Gloves, type N95 (US)