F8305

FRK (208-end), active, GST tagged human

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonim(y): GTK, PTK5, RAK
• NACRES: NA.32
• UNSPSC Code: 12352204
• Specific activity: 847-1147 nmol/min·mg
• Assay: ≥70% (SDS-PAGE)
• Recombinant: expressed in baculovirus infected Sf9 cells

Właściwości

• recombinant: expressed in baculovirus infected Sf9 cells
• Quality Segment: 200
• product line: PRECISIO^® Kinase
• assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: 847-1147 nmol/min·mg
• mol wt: ~60 kDa
• UniProt accession no.: P42685
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... FRK(2444)

Opis

General description

FRK (fyn-related kinase) is a tyrosine kinase, with a molecular weight of 54kDa. It contains SH2 and SH3 (src homology) domains. It is predominantly expressed in tissues of epithelial origin such as, liver, kidney, breast and colon. Its SH2 domain contains a putative bipartite nuclear localization signal.

Biochem/physiol Actions

FRK (fyn-related kinase) controls growth by phosphorylating perinuclear proteins. It inhibits cell growth both in a pRb (retinoblastoma)-dependent and independent manner. It cause cell cycle arrest at G1 phase in breast cancer cells. Certain mutations in this gene lead to its constitutive activation, as seen in hepatocellular adenomas. Its up-regulation in gliomas prevents both invasiveness and migration of glioma cells. This is attributable to its promotion of N-cadherin/β-catenin complex formation.

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Ta strona może zawierać tekst przetłumaczony maszynowo.

Informacja o środkach bezpieczeństwa

• Klasa składowania: 10 - Combustible liquids
• Co się dzieje?: WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• PPE (środki ochrony indywidualnej): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)