SML4478

Difluorocarboxyfluorescein-488 NHS Ester 5-isome

≥95% (HPLC)

• Synonim(y): 1-[[(2′,7′-difluoro-3′,6′-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9′-[9H]xanthen]-5-yl)carbonyl]oxy]-2,5-pyrrolidinedione, 2′,7′-difluoro-3′,6′-dihydroxy-3-oxo-spiro[isobenzofuran-1(3H),9′-[9H]xanthene]-5-carboxylic acid 2,5-dioxo-1-pyrrolidinyl ester, 2-(2,7-Difluoro-6-hydroxy-3-oxo-3H-xanthen-9-yl)-5-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)benzoic acid succinimidyl ester, DFCF-488 NHS Dye
• Wzór empiryczny (zapis Hilla): C₂₅H₁₃F₂NO₉
• Numer CAS: 198139-51-4
• Masa cząsteczkowa: 509.37
• UNSPSC Code: 12352200
• Assay: ≥95% (HPLC)
• Form: powder

Właściwości

• Quality Segment: 100
• assay: ≥95% (HPLC)
• form: powder
• color: light orange to dark orange
• solubility: DMSO: 2 mg/mL, clear
• storage temp.: -10 to -25°C

Opis

Biochem/physiol Actions

2‘,7‘-Difluorofluorescein-5-carboxy-NHS ester 488 (DFCF-488, NHS), is an amine-reactive fluorescent dye with a high molar extinction coefficient (ε = 72,000 M⁻¹cm⁻¹), enabling efficient covalent conjugation to antibodies, peptides, proteins, and nucleic acid tracers at pH 7.5-8.5 or lower. With a pKa of ~4.7 and λₑₓ/λₑₘ ~ 490/520 nm, DFCF-488 offers high quantum yield, photostability, and pH-independent fluorescence in physiological conditions, making it ideal for calcium monitoring and pH tracking in acidic organelles and pathways. DFCF-488 conjugates support diverse applications, including live-cell imaging, FRET-based biosensing, high-throughput screening, and immunofluorescence, ensuring reliable performance for cellular labeling and detection.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable