BPS50030

HDAC Developer (2X)

• Synonym(s): buffers
• UNSPSC Code: 41105331
• eCl@ss: 32160703

Properties

• manufacturer/tradename: BPS Bioscience
• storage temp.: -65 to -96°C

Description

General description

HDAC assay developer is an essential reagent for signal generation in HDAC assays performed with any of BPS Bioscience′s HDAC fluorimetric substrates or assay kits. An appropriate fluorimetric substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the HDAC assay developer produces a fluorophore. Trichostatin A is included as an ′inhibitor stop′ for class I and II HDACs.Datasheet

Application

Study of HDAC kinetics, regulation and inhibitor sensitivity. Ideal for drug discovery and HTS applications and cell-based HDAC assays.


Datasheet and Protocols may be found here.

Preparation Note

A 2x concentrate. Product is to be used (undiluted) at 1:1 ratio with HDAC reaction.

Analysis Note

Avoid freeze/thaw cycles. Use product undiluted.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Codes: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable