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Merck

10419

8-Anilino-1-naphthalenesulfonic acid hemimagnesium salt hydrate

suitable for fluorescence, ≥95.0% (T)

Synonym(s):

1,8-ANS magnesium salt, ANSA magnesium salt, Magnesium 8-anilino-1-naphthalenesulfonate hydrate, N-Phenyl peri acid magnesium salt

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About This Item

Empirical Formula (Hill Notation):
C16H12Mg0.5NO3S · xH2O
CAS Number:
18108-68-4
Molecular Weight:
310.49 (anhydrous basis)
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
329749053
EC Number:
242-004-7
Beilstein/REAXYS Number:
4834623
MDL number:
MFCD16661224

Key Documents

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Product Name

8-Anilino-1-naphthalenesulfonic acid hemimagnesium salt hydrate, suitable for fluorescence, ≥95.0% (T)

InChI key

FBANPIUXAXTAFX-UHFFFAOYSA-L

InChI

1S/2C16H13NO3S.Mg.H2O/c2*18-21(19,20)15-11-5-7-12-6-4-10-14(16(12)15)17-13-8-2-1-3-9-13;;/h2*1-11,17H,(H,18,19,20);;1H2/q;;+2;/p-2

SMILES string

O.[Mg++].[O-]S(=O)(=O)c1cccc2cccc(Nc3ccccc3)c12.[O-]S(=O)(=O)c4cccc5cccc(Nc6ccccc6)c45

assay

≥95.0% (T)

form

powder

technique(s)

titration: suitable

solubility

hot water: 50 mg/mL, green

λmax

465-475 nm

fluorescence

λex 388 nm; λem 470 nm in 0.1 M Tris, 0.2 M KCl, pH 9.0, BSA

suitability

suitable for fluorescence

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Features and Benefits

Fluorescent probe for the estimation of the CMC of surfactants.

General description

ANSA (8-Anilino-1-naphthalenesulfonic acid) is a hydrophobic polarity sensitive fluorescent dye useful as a site probe to detect conformational changes in cell and micelle membranes and molecules such as proteins. ANSA is also used as a fluorescent probe for the estimation of the critical micelle concentration (CMC) of surfactants.

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN2524
BCCM6782
BCCL1990
BCCK8670
BCCK2567

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M Bertsch et al.
Analytical biochemistry, 313(2), 187-195 (2003-02-28)
Hydrophobic sites on the surface of protein molecules are thought to have important functional roles. The identification of such sites can provide information about the function and mode of interaction with other cellular components. While the fluorescence enhancement of polarity-sensitive
J L Jensen et al.
Cellular and molecular biology (Noisy-le-Grand, France), 46(3), 685-696 (2000-06-29)
We have studied the metal-catalyzed oxidation (MCO) of brain-derived neurotrophic factor (BDNF) with regard to target sites and potential conformational changes of the protein. The exposure of BDNF to three different levels of ascorbate/Cu(II)/O2 [20 microM Cu(II), 2 mM ascorbate
Rapid fluorescent "staining" of nondenatured protein bands in agar and polyacrylamide gels.
S T Nerenberg et al.
Analytical biochemistry, 43(2), 564-574 (1971-10-01)
C D Peters et al.
Biochemistry and cell biology = Biochimie et biologie cellulaire, 75(1), 55-61 (1997-01-01)
Protein aggregation is believed to be due to conformers that expose hydrophobic clusters that promote protein association. Such conformers can be detected using a fluorescent probe like 8-anilino 1-naphthalenesulfonic acid (ANSA). Here we show that urease exposed to 1.0 M
F-O McDuff et al.
Biochemistry and cell biology = Biochimie et biologie cellulaire, 82(2), 305-313 (2004-04-03)
Canavalia ensiformis (jack bean) alpha-urease is a hexameric protein characterized by a complex denaturation mechanism. In previous papers, we have shown that a hydrophobic 8-anilino-1-naphthalenesulfonic acid (ANSA) binding conformer could be populated in a moderate concentration of denaturant. This state
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