Merck

Guanidine methods for total RNA preparation.

Current protocols in molecular biology (2008-02-12)
R E Kingston, P Chomczynski, N Sacchi
ABSTRACT

Three different methods for RNA preparation using guanidine are presented in this unit--a single-step isolation method employing liquid-phase separation to selectively extract total RNA from tissues and cultured cells and two methods that rely on a CsCl step gradient to isolate total RNA.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Sodium acetate, anhydrous, ReagentPlus®, ≥99.0%
Sigma-Aldrich
Sodium acetate, ACS reagent, ≥99.0%
Sigma-Aldrich
Sodium Acetate Anhydrous, >99%, FCC, FG