FPTase, Rat, Recombinant, E. coli

Name: FPTase, Rat, Recombinant, <i>E. coli</i>
Brand: MM

A heterodimeric enzyme that catalyzes the transfer of a 15-carbon isoprenoid group to a variety of cellular proteins including Ras.

Synonym(s):

Cathepsin L2, Cathepsin U, Farnesyl Proteintransferase, FTase

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About This Item

UNSPSC Code:

12352202

NACRES:

NA.77

Key Documents

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recombinant

expressed in E. coli

Quality Level

200

Assay

≥85% (SDS-PAGE)

form

liquid

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

−70°C

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This Item	539514
Sigma-Aldrich 344145 FPTase, Rat, Recombinant, E. coli	Sigma-Aldrich 539514 Protein Phosphatase, Lambda, Recombinant, E. coli
assay ≥85% (SDS-PAGE)	assay ≥95% (SDS-PAGE)
form liquid	form liquid
recombinant expressed in E. coli	recombinant expressed in E. coli
shipped in wet ice	shipped in wet ice
storage temp. −70°C	storage temp. −70°C
storage condition OK to freeze, avoid repeated freeze/thaw cycles	storage condition OK to freeze, avoid repeated freeze/thaw cycles

General description

M.W. α-subunit: ~48,000; β-subunit: ~46,000

Recombinant, rat FPTase expressed in E. coli. A heterodimeric enzyme that catalyzes the transfer of a 15-carbon isoprenoid group to a C-terminal cysteine in a variety of cellular proteins, including Ras. Recognizes the CaaX motif where the farnesylated cysteine (C) is followed by two aliphatic amino acids (aa) and either leucine or serine (X). Expressed in E. coli as an untagged recombinant protein.

Biochem/physiol Actions

1 pmol of FPTase will transfer 1 pmol of farnesyl to H-Ras in 15 min at 37°C.

Physical form

In 25 mM HEPES, pH 7.2, 40 mM NaCl, 5µM ZnCl2, and 1mM TECP

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Hightower, K.E., et al. 2001. Biochem. J.360, 625.
Zimmerman, K.K., et al. 1998. Protein Expr. Purif.14, 395.
Reiss, Y., et al. 1990. Cell62, 81.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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