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SCR060

Human Neural Stem Cell Characterization Kit

The Human Neural Stem Cell Characterization Kit contains three molecular markers, Nestin, Sox 2 & Musashi that are frequently used to identify neural stem/progenitor cells.

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84

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Quality Level

100

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

cell culture | stem cell: suitable
immunocytochemistry: suitable

input

sample type neural stem cell(s)

shipped in

dry ice

General description

The Human Neural Stem Cell Characterization Kit (Catalog Number SCR060) contains three molecular markers, Nestin (Lendahl, et al., 1990) and Sox 2 (Graham, et al., 2003) and Musashi (Sakakibara, et al., 1996) that are frequently used to identify neural stem/progenitor cells along with more differentiated lineage markers including bIII-tubulin for neurons, GFAP for astrocytes and O1 for oligodendrocytes. Mouse and rabbit immunoglobulins for the assessment of background staining are also included. All of the antibodies provided in the kit have been tested and optimized for use in immunocytochemistry on human neural stem cells. We recommend that the Human Neural Stem Cell Characterization Kit be used in conjunction with differentiation assays that demonstrate multipotentiality of the starting cell population.

Application

Research Category
Stem Cell Research
The Human Neural Stem Cell Characterization Kit contains three molecular markers, Nestin, Sox 2 & Musashi that are frequently used to identify neural stem/progenitor cells.

Preparation Note

When stored at the recommended storage conditions (refer to Kit Components), components are stable up to the expiration date. Do not expose to elevated temperatures. Discard any remaining reagents after the expiration date.

Other Notes

Mouse anti-Nestin, 50ug

Rabbit anti-Sox-2, 20ug

Rabbit anti-Musashi, 50ug

Mouse anti-bIII Tubulin, 50ul

Rabbit anti-GFAP, 50ul

Mouse anti-Oligodendrocyte marker O1, 20ug

Mouse IgM, 50ug

Mouse IgG, 100ug

Rabbit IgG, 100ug

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

Certificates of Analysis (COA)

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S Sakakibara et al.
Developmental biology, 176(2), 230-242 (1996-06-15)
There is increasing interest in the role of RNA-binding proteins during neural development. Drosophila Musashi is one of the neural RNA-binding proteins essential for neural development and required for asymmetric cell divisions in the Drosophila adult sensory organ development. Here
Jin-Seon Song et al.
Differentiation; research in biological diversity, 77(1), 29-37 (2009-03-14)
Regenerating human tooth ex vivo and biological repair of dental caries are hampered by non-viable odontogenic stem cells that can regenerate different tooth components. Odontoma is a developmental dental anomaly that may contain putative post-natal stem cells with the ability
Sakthidasan Jayaprakash et al.
Molecular and cellular biochemistry, 461(1-2), 171-182 (2019-08-21)
The BAF complex (SWI/SNF) is an ATP-dependent chromatin remodeler that adapts the structural organization of the chromatin. Despite a growing understanding of the composition of BAF in different cell types, the interaction network within the BAF complex is poorly understood.
Hava Karsenty Avraham et al.
British journal of pharmacology, 171(2), 468-479 (2013-10-24)
HIV-1 glycoprotein Gp120 induces apoptosis in rodent and human neurons in vitro and in vivo. HIV-1/Gp120 is involved in the pathogenesis of HIV-associated dementia (HAD) and inhibits proliferation of adult neural progenitor cells (NPCs) in glial fibrillary acidic protein (GFAP)/Gp120 transgenic
U Lendahl et al.
Cell, 60(4), 585-595 (1990-02-23)
Multipotential CNS stem cells receive and implement instructions governing differentiation to diverse neuronal and glial fates. Exploration of the mechanisms generating the many cell types of the brain depends crucially on markers identifying the stem cell state. We describe a
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