The kit does not have specified lower and upper detection limits for enzyme activity. The enzyme activity needs to be empirically determined using varying enzyme amounts.
CS0780
β-N-Acetylglucosaminidase Assay Kit
sufficient for 50 reactions (1 mL), sufficient for 500 reactions (100 μL)
Synonym(s):
Beta-NAG Activity Assay Kit
Sign In to View Organizational & Contract Pricing.
Select a Size
1 KIT
$815.00
About This Item
UNSPSC Code:
12161503
EC Number:
222-398-7
NACRES:
NA.84
Skip To
Product Name
β-N-Acetylglucosaminidase Assay Kit, sufficient for 50 reactions (1 mL), sufficient for 500 reactions (100 μL)
usage
sufficient for 50 reactions (1 mL)
sufficient for 500 reactions (100 μL)
shipped in
wet ice
storage temp.
2-8°C
Quality Level
Gene Information
human ... NAGLU(4669)
Related Categories
1 of 4
This Item | CS0720 | CS1090 | CS0170 |
|---|---|---|---|
| Gene Information human ... NAGLU(4669) | Gene Information human ... CS(1431) | Gene Information human ... HMGCR(3156) | Gene Information human ... TXNRD1(7296), TXNRD2(10587), TXNRD3(114112) |
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
| usage sufficient for 50 reactions (1 mL), sufficient for 500 reactions (100 μL) | usage kit sufficient for 100 reactions (using a 1 ml cuvette), kit sufficient for 480 reactions (using 96 multiwell plates) | usage sufficient for 100 assays (200 μL), sufficient for 30 assays (1 mL) | usage kit sufficient for 100 assays (1 mL) |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −70°C | storage temp. −20°C |
| shipped in wet ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
Analysis Note
The kit assay is based on the hydrolysis of NAG substrate (NP-GLcNAc) by the enzyme. The enzymatic hydrolysis of the substrate liberates p-nitrophenylate ion. The reaction product is detected colorimetrically at 405 nm.
Application
The kit provides the reagents required for a fast and convenient detection of β-N-Acetylglucosaminidase activity in cell lysates, tissue homogenates or purified enzyme. It is a useful tool for the detection of lysosomes in fractionated cell/tissue samples.
Biochem/physiol Actions
N-acetyl-D-glucosaminidase (NAG) in mammals is a lysosomal enzyme, which takes part in the intracellular degradation of glycolipids and glycoproteins. High activities of this enzyme have been detected in human kidney, lung and liver lysosomes. Elevated levels of serum NAG are associated with certain forms of cancer.
related product
Product No.
Description
Pricing
signalword
Warning
hcodes
Hazard Classifications
Carc. 2 - Eye Irrit. 2 - STOT RE 2 Oral
target_organs
Liver,Kidney
Storage Class
11 - Combustible Solids
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Shanshan Wang et al.
Biochemical and biophysical research communications, 483(1), 45-51 (2017-01-09)
Activation of endothelial cells plays a key role in septic acute kidney injury (AKI). This study investigated the role of miRNA in endothelial-induced tubular cell injury in sepsis. Circulating endothelial cells (CECs) from septic AKI, non-septic AKI, septic non-AKI patients
Miguel A Lanaspa et al.
Journal of the American Society of Nephrology : JASN, 25(11), 2526-2538 (2014-05-31)
Diabetes is associated with activation of the polyol pathway, in which glucose is converted to sorbitol by aldose reductase. Previous studies focused on the role of sorbitol in mediating diabetic complications. However, in the proximal tubule, sorbitol can be converted
Qiuyuan Yin et al.
The Journal of cell biology, 219(8) (2020-07-15)
Lysosomes are degradation and signaling organelles that adapt their biogenesis to meet many different cellular demands; however, it is unknown how lysosomes change their numbers for cell division. Here, we report that the cyclin-dependent kinases CDK4/6 regulate lysosome biogenesis during
Di Chen et al.
Developmental cell, 55(5), 588-602 (2020-12-09)
Liquid-liquid phase separation (LLPS) compartmentalizes transcriptional condensates for gene expression, but little is known about how this process is controlled. Here, we showed that depletion of IPMK, encoding inositol polyphosphate multikinase, promotes autophagy and lysosomal function and biogenesis in a
Xiaoting Chen et al.
Journal of cellular and molecular medicine, 23(6), 4290-4300 (2019-04-12)
Impaired autophagic degradation of intracellular lipids is causally linked to the development of non-alcoholic steatohepatitis (NASH). Pharmacological agents that can restore hepatic autophagic flux could therefore have therapeutic potentials for this increasingly prevalent disease. Herein, we investigated the effects of
Articles
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
亚细胞组分离心分离是一种常用技术，广泛适用于多种类型的细胞和组织。由于不同细胞器的大小、形状和密度不同，因此通过离心，可从温和均质的样品中轻松分离和纯化细胞器组分。
Related Content
-
What are the lower and upper limits of enzyme activity that the CS0780 B-N-Acetylglucosaminidase Assay Kit can measure?
1 answer-
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service