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D4522

Deoxyribonucleic acid from calf thymus

Type XV, Activated, lyophilized powder

Synonym(s):

Calf Thymus DNA, DNA from calf thymus, Thymonucleic acid

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$71.30

5 MG

$264.00

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$967.00

$71.30

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About This Item

CAS Number:
91080-16-9
UNSPSC Code:
41106310
eCl@ss:
32160414
EC Number:
293-507-3
NACRES:
NA.52
MDL number:
MFCD00151698

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Product Name

Deoxyribonucleic acid from calf thymus, Type XV, Activated, lyophilized powder

biological source

bovine pancreas

grade

Molecular Biology

type

Type XV

form

lyophilized powder

solubility

water: slightly soluble

storage temp.

−20°C

Quality Level

200

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Show Differences

1 of 4

This Item
D4764D8899D8273
Deoxyribonucleic acid from calf thymus Type XV, Activated, lyophilized powder

Sigma-Aldrich

D4522

Deoxyribonucleic acid from calf thymus

Deoxyribonucleic acid from calf thymus Genomic, unsheared

Sigma-Aldrich

D4764

Deoxyribonucleic acid from calf thymus

Deoxyribonucleic acid, single stranded from calf thymus lyophilized powder

Sigma-Aldrich

D8899

Deoxyribonucleic acid, single stranded from calf thymus

Deoxyribonucleic acid−cellulose single-stranded from calf thymus DNA lyophilized powder

Sigma-Aldrich

D8273

Deoxyribonucleic acid−cellulose single-stranded from calf thymus DNA

grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

grade

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

type

Type XV

type

-

type

-

type

-

Application

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard to determine DNA content in gels[1] and for DNA quantification by fluorescent assay.[2]

Features and Benefits

• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA

General description

High quality double-stranded template DNA isolated from the thymus of male and female calves.

Preparation Note

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.
One mg DNA is approx. 20 A260 units.
Prepared from highly polymerized calf thymus DNA and nicked with DNase using the method of Aposhian and Kornberg.[3]

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Storage Class

11 - Combustible Solids

wgk

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flash_point_f

Not applicable

flash_point_c

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ppe

Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000425610
0000425610
0000402444
0000402444
0000402449

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Brian D Holt et al.
Advanced healthcare materials, 5(23), 3056-3066 (2016-12-08)
Synthetic biomaterials are poised to transform medicine; however, current synthetic options have yet to ideally recapitulate the desirable properties of native tissue. Thus, the development of new synthetic biomaterials remains an active challenge. Due to its excellent properties, including electrical
Benjamin D Dickson et al.
Molecules (Basel, Switzerland), 24(8) (2019-04-24)
Poly(ADP-ribose)polymerase (PARP) inhibitors (PARPi) have recently been approved for the treatment of breast and ovarian tumors with defects in homologous recombination repair (HRR). Although it has been demonstrated that PARPi also sensitize HRR competent tumors to cytotoxic chemotherapies or radiotherapy
Enzymatic synthesis of deoxyribonucleic acid. IX. The polymerase formed after T2 bacteriophage infection of Escherichia coli: a new enzyme.
H V APOSHIAN et al.
The Journal of biological chemistry, 237, 519-525 (1962-02-01)
Anastasia S Singatulina et al.
Cell reports, 27(6), 1809-1821 (2019-05-09)
PARP-1 synthesizes long poly(ADP-ribose) chains (PAR) at DNA damage sites to recruit DNA repair factors. Among proteins relocated on damaged DNA, the RNA-binding protein FUS is one of the most abundant, raising the issue about its involvement in DNA repair.
Juan Antonio Aguilar-Pimentel et al.
Scientific reports, 10(1), 18334-18334 (2020-10-29)
Sex steroids, such as estrogens and androgens, are important regulators of the humoral immune response. Studies in female mice have demonstrated that alteration of circulating estrogen concentration regulates antibody-mediated immunity. As males have normally little endogenous estrogen, we hypothesized that
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Questions

1–7 of 7 Questions  

  1. Could you please confirm whether this product is suitable to be used as a DNA standard for generating a calibration curve (0–200 ng/µL) in such applications? DNA quantification using the Hoechst 33258 dye in a fluorescence-based assay.

    1 answer

    1. The molecular weight for D4522 is reported to be between 10-15 million Daltons prior to activation (nicking). 15,000 kilodaltons x (1kb/660 kilodaltons) = 22.72 kb (on average). The average number of base pairs is not measured, and therefore, this calculation is an estimate. We do not have a molecular weight measurement after it's been nicked, but it is expected to be a range below 22.72 kB. We do not have comparison data to inform us of how they may respond differently as a standard curve for a fluorescent assay. We would suggest checking to see if the mechanics behind your assay are length dependent or could be influenced by the ends being nicked or not. Products like D4764 that are deemed stable for long periods of time do not have a noted expiration or retest date.

      Helpful?

  2. Could you provide the number of base pairs for the D4522 product? For example, is it around 20kb?

    1 answer

    1. The reported molecular weight is between 10-15 million Daltons before activation (nicking), primarily as double-stranded DNA. A double-stranded DNA of 1 kb in length has a molecular weight of 660 kiloDalton. A size of 15 million daltons is equivalent to 15,000 kilodaltons. When multiplied by (1kb/660 kilodaltons), it equals 22.72 kb on average. It's important to note that the average number of base pairs is not measured, so this calculation provides an estimate.

      Helpful?

  3. What is the molecular weight of Product D4522, Deoxyribonucleic acid?

    1 answer

    1. The molecular weight is reported to be between 10-15 million Daltons prior to activation (nicking).

      Helpful?

  4. Is Product D4522, Deoxyribonucleic acid, single or double stranded?

    1 answer

    1. Even though it is activated or "nicked" DNA, we have found that it is primarily double stranded.

      Helpful?

  5. What is the solution stability of Product D4522, Deoxyribonucleic acid?

    1 answer

    1. Solutions of deoxyribonucleic acid have been stored successfully for several months at 4°C, in 10 mM Tris, pH 7.5 - 8.0, with 1 mM EDTA and without a bacteriostatic agent.  At low concentrations (25 μg/mL), DNA tends to absorb onto the surfaces of plastic tubes.  It is not recommended to store deoxyribonucleic acid in alkaline solutions since it tends to degrade at pH greater than 8.0.

      Helpful?

  6. Is BSA (bovine serum albumin) used during the method of preparation for Product D4522, Deoxyribonucleic acid?

    1 answer

    1. During the method of preparation, when deoxyribonucleic acid is nicked, BSA is added to the solution.  This is followed by an extraction method to remove all exogenous protein. We do not, however, claim this deoxyribonucleic acid is 100% clean of all proteins (including BSA).

      Helpful?

  7. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

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