40TST can be used for 40 reactions.
DUO92004
Duolink® In Situ PLA® Probe Anti-Mouse MINUS
Affinity purified Donkey anti-Mouse IgG (H+L)
Duolink® In Situ PLA® Probe Anti-Mouse MINUS
Synonym(s):
in situ Proximity Ligation Assay reagent, Protein Protein Interaction Assay reagent
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biological source
donkey (polyclonal)
Quality Level
antibody form
affinity purified immunoglobulin (secondary antibody)
antibody product type
primary antibodies
product line
Duolink®
species reactivity
mouse
technique(s)
immunofluorescence: suitable
proximity ligation assay: suitable
suitability
suitable for brightfield
suitable for fluorescence
shipped in
wet ice
storage temp.
2-8°C
1 of 4
This Item | DUO92001 | DUO92002 | DUO92003 |
|---|---|---|---|
| antibody form affinity purified immunoglobulin (secondary antibody) | antibody form affinity purified immunoglobulin (secondary antibody) | antibody form affinity purified immunoglobulin (secondary antibody) | antibody form affinity purified immunoglobulin (secondary antibody) |
| species reactivity mouse | species reactivity mouse | species reactivity rabbit | species reactivity goat |
| biological source donkey (polyclonal) | biological source donkey (polyclonal) | biological source donkey (polyclonal) | biological source donkey (polyclonal) |
| technique(s) immunofluorescence: suitable, proximity ligation assay: suitable | technique(s) immunofluorescence: suitable, proximity ligation assay: suitable | technique(s) immunofluorescence: suitable, proximity ligation assay: suitable | technique(s) immunofluorescence: suitable, proximity ligation assay: suitable |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
General description
Duolink® In Situ PLA® Probe Anti-Mouse MINUS contains affinity purified donkey anti-mouse IgG (H+L), which reacts with whole molecule mouse IgG and also reacts with the light chains of other mouse immunoglobulins.
Application
Duolink®proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.[1][2][3]
This product can be applied to both the Duolink® In Situ Fluorescence Protocol and the Duolink® In Situ Brightfield Protocol depending on the detection reagents used.
To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.HRP is also available for brightfield detection.
This product can be applied to both the Duolink® In Situ Fluorescence Protocol and the Duolink® In Situ Brightfield Protocol depending on the detection reagents used.
To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.HRP is also available for brightfield detection.
Specificity
PLA probe anti-Mouse reacts with whole molecule Mouse IgG and the light chains of other Mouse immunoglobulin?s. The PLA probe anti-Mouse may cross-react with Rat antibodies, but has minimal cross reactivity with bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, rabbit, and sheep serum proteins. A PLUS probe of a different species must be used simultaneously with this product. See our Product Selection Guide for more information.
Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink®PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects
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PLA probe anti-Mouse reacts with whole molecule Mouse IgG and the light chains of other Mouse immunoglobulin?s. The PLA probe anti-Mouse may cross-react with Rat antibodies, but has minimal cross reactivity with bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, rabbit, and sheep serum proteins. A PLUS probe of a different species must be used simultaneously with this product. See our Product Selection Guide for more information.
Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink®PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects
View full Duolink® product list
Features and Benefits
- No overexpression or genetic manipulation required
- High specificity (fewer false positives)
- Single molecule sensitivity due to rolling circle amplification
- Relative quantification possible
- No special equipment needed
- Quicker and simpler than FRET
- Increased accuracy compared to co-IP
- Publication-ready results
Preparation Note
Storage and Stability: Store the PLA Probe Anti-Mouse MINUS at 2–8°C. Do not store the diluted PLA Probe Anti-Mouse MINUS solution.
Other Notes
This product is comprised of the following:
- 5x PLA Probe Anti-Mouse MINUS - Donkey anti-mouse secondary antibody conjugated to oligonucleotide MINUS
- 1x Blocking Solution - Reagent for blocking of the sample
- 1x Antibody Diluent - For dilution of PLA probes and primary antibodies
Legal Information
Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
PLA is a registered trademark of Merck KGaA, Darmstadt, Germany
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Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 2 - Skin Sens. 1
Storage Class Code
12 - Non Combustible Liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Proximal Ligation Assay (PLA) on Lung Tissue and Cultured Macrophages to Demonstrate Protein-protein Interaction.
Mendez R and Santanu B
Bio-protocol, 7(21) (2017)
Novel function of ceramide for regulation of mitochondrial ATP release in astrocytes.
Kong J N, et al.
Journal of Lipid Research, M081877-M081877 (2018)
Interaction of the Mouse Polyomavirus Capsid Proteins with Importins Is Required for Efficient Import of Viral DNA into the Cell Nucleus.
Soldatova I, et al.
Viruses, 10(4), 165-165 (2018)
Roberto Di Maio et al.
Science translational medicine, 10(451) (2018-07-27)
Missense mutations in leucine-rich repeat kinase 2 (LRRK2) cause familial Parkinson's disease (PD). However, a potential role of wild-type LRRK2 in idiopathic PD (iPD) remains unclear. Here, we developed proximity ligation assays to assess Ser1292 phosphorylation of LRRK2 and, separately
Pengcheng Zhu et al.
Cancer cell, 19(3), 401-415 (2011-03-15)
Cancer is a leading cause of death worldwide. Tumor cells exploit various signaling pathways to promote their growth and metastasis. To our knowledge, the role of angiopoietin-like 4 protein (ANGPTL4) in cancer remains undefined. Here, we found that elevated ANGPTL4
Articles
包括建议和技巧、常见问题解答和基本故障排除的支持信息。
Learn how Proximity Ligation Assay technology works and how the protein-protein interaction control kit can confirm in situ detection of EGF-induced EGFR-HER2 dimerization.
Support information including tips and tricks, frequently asked questions, and basic troubleshooting.
Proteins interact with various molecules, including other proteins, to fulfill complex cellular functions within biological systems.
Protocols
Duolink® PLA reagents enable brightfield detection and quantification of proteins and interactions in tissue samples.
使用明场显微镜和Duolink® PLA试剂用于检测和定量组织和细胞样品中的个体蛋白质、蛋白质修饰和蛋白质相互作用。
本实验方案描述如何使用Duolink® PLA邻位连接试剂，通过流式细胞术检测细胞群内的单个蛋白质、蛋白修饰和蛋白相互。
Related Content
View an automated protocol for Duolink® assays on the AAW™ automated assay workstation and see results comparing manual vs automated runs.
利用邻位连接技术对蛋白质互作、翻译后修饰和低表达蛋白进行检测、定量和成像应用。
Applications to detect, quantify and visualize protein-protein interactions, post-translational modifications and low expression protein detection using proximity ligation assay
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