NA2100

GenElute^™ Bacterial Genomic DNA Kits

sufficient for 10 purifications

• Synonym(s): Bacterial Genomic DNA, Gen Elute
• NACRES: NA.55
• UNSPSC Code: 41105501

Properties

• Product Name: GenElute^™ Bacterial Genomic DNA Kits, sufficient for 10 purifications
• usage: sufficient for 10 purifications
• technique(s): DNA purification: suitable
• storage temp.: 15-25°C
• Quality Level: 200

Description

Application

The purified bacterial genomic DNA is ready for downstream applications such as:

• restriction endonuclease digestions
• PCR
• Southern blots
• cloning

Biochem/physiol Actions

The bacteria are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes the DNA to bind when the lysate is spun through a silica membrane into a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution.

The expected yield of genomic DNA will vary depending on the cell density of the bacterial culture and the bacterial species and strain used. DNA purified with the GenElute kit has an A₂₆₀/A₂₈₀ ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Features and Benefits

• Starting material: Up to 1.5 mL of culture
• Expected yield: Up to 20 μg
• Elution volume: 400 μl
• Time required: 70 - 120 min
• A₂₆₀/A₂₈₀ ratio: 1.6 - 1.9
• No phenol, chloroform, or ethanol precipitation required

• Typical DNA yields of 15 μg - 20 μg
• Protocols provided for Gram + and Gram - bacteria
• High quality genomic DNA in less than 2 hours
• Purified DNA has an A₂₆₀/A₂₈₀ ratio between 1.6 and 1.9

General description

The GenElute Bacterial Genomic DNA kit provides a simple and convenient way to isolate pure genomic DNA from gram-negative bacteria. For most gram-positive bacteria, the kit must be used in conjunction with the optional lysozyme (L4919) to effectively lyse the thick peptidoglycan cell walls. A Gram-Positive Lysis Solution is provided as a diluent for preparing the lysozyme stock solutions.

The kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.

Click here to view the kit protocol

Other Notes

For additional information, please see www.sigma-aldrich.com/genomicdna.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• pictograms: GHS02,GHS08,GHS05,GHS07
• signalword: Danger
• hcodes: H226,H290,H302,H315,H319,H334,H335,H336
• pcodes: P210 - P233 - P234 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Central nervous system, Respiratory system
• Storage Class: 3 - Flammable liquids