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SAB4501344

Anti-Guanylate Cyclase β antibody produced in rabbit

affinity isolated antibody

Synonym(s):

GCS-β-1, GCS-β-3, GCYB1, GUCY1B1, guanylate cyclase soluble subunit β-1

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100 μG

$621.00

$621.00

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

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biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 70 kDa

species reactivity

human, rat, mouse

concentration

~1 mg/mL

technique(s)

ELISA: 1:5000
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000

NCBI accession no.

Q02153

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GUCY1B3(2983)

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Sigma-Aldrich

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biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

Quality Level

100

Quality Level

100

Quality Level

200

Quality Level

200

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

saturated ammonium sulfate (SAS) precipitated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

technique(s)

ELISA: 1:5000, western blot: 1:500-1:1000, immunohistochemistry: 1:50-1:100

technique(s)

immunoblotting: 0.04-0.4 μg/mL, immunohistochemistry: 1:20-1:50

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 using trypsin-digested human, bovine and mouse heart tissue, immunoprecipitation (IP): 2-3 μg using 60-120 μg of a cytosolic fraction of rat brain, western blot: 1:2,000 using cytosolic fraction of rat brain

technique(s)

immunohistochemistry: 1:50-1:100, indirect ELISA: 1:1000

mol wt

antigen 70 kDa

mol wt

-

mol wt

antigen 70 kDa

mol wt

-

General description

Anti-Guanylate Cyclase β Antibody detects endogenous levels of total Guanylate Cyclase β protein.

Immunogen

The antiserum was produced against synthesized peptide derived from human Guanylate Cyclase beta.

Immunogen Range: 10-59

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
310219

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Rafael S Fais et al.
Scientific reports, 9(1), 16224-16224 (2019-11-09)
NLRP3 plays a role in vascular diseases. Corpora cavernosa (CC) is an extension of the vasculature. We hypothesize that NLRP3 plays a deleterious role in CC relaxation. Male C57BL/6 (WT) and NLRP3 deficient (NLRP3-/-) mice were used. Intracavernosal pressure (ICP/MAP)
J Sevilla-Montero et al.
Free radical biology & medicine, 193(Pt 1), 9-22 (2022-09-30)
Chronic obstructive pulmonary disease (COPD), whose main risk factor is cigarette smoking (CS), is one of the most common diseases globally. Some COPD patients also develop pulmonary hypertension (PH), a severe complication that leads to premature death. Evidence suggests reactive
Kálmán Benke et al.
Scientific reports, 7(1), 14232-14232 (2017-10-29)
While heart transplantation (HTX) is the definitive therapy of heart failure, donor shortage is emerging. Pharmacological activation of soluble guanylate cyclase (sGC) and increased cGMP-signalling have been reported to have cardioprotective properties. Gemfibrozil has recently been shown to exert sGC

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