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504955

Discovery® C18 (5 µm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

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$1,390.00

$1,390.00

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501

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Product Name

Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

Agency

suitable for USP L1

feature

endcapped

manufacturer/tradename

Discovery®

extent of labeling

12% Carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

15 cm × 4.6 mm

surface area

300 m2/g

surface coverage

3 μmol/m2

impurities

<10 ppm metals

matrix

silica gel, high purity, spherical particle platform

matrix active group

C18 (octadecyl) phase

particle size

5 μm

pore size

180 Å

operating pH range

2-8

application(s)

food and beverages

separation technique

reversed phase

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This Item
50494750497150494721
Discovery&#174; C18 HPLC Column 5&#160;&#956;m particle size, L × I.D. 15&#160;cm × 4.6&#160;mm

Supelco

504955

Discovery® C18 HPLC Column

Discovery&#174; C18 HPLC Column 5&#160;&#956;m particle size, L × I.D. 5&#160;cm × 4.6&#160;mm

Supelco

504947

Discovery® C18 HPLC Column

Discovery&#174; C18 HPLC Column 5&#160;&#956;m particle size, L × I.D. 25&#160;cm × 4.6&#160;mm

Supelco

504971

Discovery® C18 HPLC Column

Discovery&#174; C18 HPLC Column 5&#160;&#956;m particle size, L × I.D. 5&#160;cm × 2.1&#160;mm

Supelco

50494721

Discovery® C18 HPLC Column

L × I.D.

15 cm × 4.6 mm

L × I.D.

5 cm × 4.6 mm

L × I.D.

25 cm × 4.6 mm

L × I.D.

5 cm × 2.1 mm

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

particle size

5 μm

particle size

5 μm

particle size

5 μm

particle size

5 μm

pore size

180 Å

pore size

180 Å

pore size

180 Å

pore size

180 Å

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

General description

Use Discovery® C18 for any method that specifies a C18. The exceptional peak shape, reproducibility, and stability make it the column of choice for all C18 methods from demanding to routine.

Features and Benefits

  • Excellent reproducibility
  • Exceptional peak shape for basic and acidic analytes
  • Stable, low-bleed LC-MS separations
  • Separation of peptides and small proteins
  • Lower hydrophobicity than many comparable C18 columns, providing faster analysis

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

related product

Product No.
Description
Pricing

used together

Product No.
Description
Pricing

505129

Discovery® C18 Supelguard Guard Cartridge, Kit, 5 μm particle size, L × I.D. 2 cm × 4 mm

G001085

HPLC Analysis of Angiotensins on Discovery® C18, application for HPLC

G000595

HPLC Analysis of Tricyclic Antidepressants in Serum on Discovery® C18 after SPE using Discovery® DSC-18, application for SPE, application for HPLC

G001056

HPLC Analysis of Barbiturates in Serum on Discovery® C18 after SPE using Discovery® DSC-18Lt, application for SPE, application for HPLC

G001635

HPLC Analysis of 3-Methylpyrazole and 4-Methylpyrazole in Urine on Discovery® C18 after SPE using Discovery® DSC-SCX, application for SPE, application for HPLC

G003764

HPLC Analysis of Furosemide (Lasix) in Horse Serum on Discovery® C18 after SPE using Discovery® DSC-18, application for SPE, application for HPLC

G005684

HPLC Analysis of Caffeine Metabolites and Analogs on Discovery® C18, application for HPLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
298515-06
298515-05
298515-04
298515-03
298515-02

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T Nagano et al.
Journal of dental research, 88(9), 823-828 (2009-09-22)
Mmp-20 and Klk4 are the two key enamel proteases. Can both enzymes process amelogenin to generate the major cleavage products that accumulate during the secretory stage of amelogenesis? We isolated Mmp-20 and Klk4 from developing pig teeth and used them
Satoru Sonoke et al.
Cancer research, 68(21), 8843-8851 (2008-11-01)
The pharmacokinetics and antitumor activity of pegylated small interfering RNA (siRNA)/cationic liposome complexes were studied after systemic administration to mice. We designed pegylated-lipid carriers for achieving increased plasma concentrations of RNA and hence improved accumulation of RNA in tumors by
Pilar Heredia-Middleton et al.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 149(4), 552-556 (2008-02-01)
Heat shock protein 70 (HSP70, 70 kDa) is the most commonly expressed protein in response to thermal stress. The extent of its expression is associated with differences in environmental temperatures. We investigated the heat shock response in red blood cells
Yuan-Qing Xia et al.
Rapid communications in mass spectrometry : RCM, 20(12), 1831-1837 (2006-05-18)
A major challenge in selecting an appropriate stationary phase for diastereomeric separation is that it is difficult to predict which of the commercially available stationary phases could achieve the required liquid chromatographic (LC) separation. This work describes the selection and
T R Shantha Kumar et al.
Journal of pharmaceutical and biomedical analysis, 38(1), 173-179 (2005-05-24)
A simple, precise and accurate isocratic reverse-phase liquid chromatography method with programmed wavelength detection has been validated to quantify DRF-4367 and Phenol red, simultaneously for application in rat in situ single pass intestinal perfusion study to assess intestinal permeability of
View All Related Papers

Articles

Determination of Tanshinone IIA and key ginsenosides

Method development for HPLC-UV quantification of tanshinone IIA and key ginsenosides in Guanxin Danshen Diwan as per the Chinese Pharmacopoeia draft.

Determination of Sucralose in Soy Sauce using HPLC-ELSD following the Chinese National Standard (GB) Method

Determination of Sucralose in Soy Sauce using HPLC-ELSD following the Chinese National Standard (GB) Method

Protocols

Separations of Tetracycline Antibiotics by Reversed Phase HPLC, Using Discovery Columns

Separations of Tetracycline Antibiotics by Reversed Phase HPLC, Using Discovery Columns

Extraction and Analysis of Lasix (Furosemide) in Horse Serum

Studies have suggested that there is a correlation between furosemide and improved racing performance, prompting the horse racing industry to monitor the use of this drug

HPLC Analysis of Barbiturates in Serum on Discovery® C18 after SPE using Discovery® DSC-18Lt

HPLC Analysis of Barbiturates in Serum on Discovery® C18 after SPE using Discovery® DSC-18Lt

HPLC Analysis of Aniline Homologs on Discovery® C18

HPLC Analysis of Aniline Homologs on Discovery® C18

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Chromatograms

Questions

1–3 of 3 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. What is the  shipping solvent for Discovery® C18 HPLC Column, 504955?

    1 answer

    1. The shipping solvent for Discovery®C18 HPLC Column, 504955 is 66% Methanol: 34% Water.

      Helpful?

  3. How can I determine the shelf life / expiration / retest date of this product?

    0 answers

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