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C1907

钙调磷酸酶来源于牛大脑

Name: 钙调磷酸酶 来源于牛大脑
Brand: SIGMA
Price: 970 SGD

lyophilized powder, ≥2,500 units/mg protein

别名:

PP2B, 磷蛋白磷酸水解酶, 蛋白磷酸酶 2B, 调节因子结合蛋白, 钙/钙调素活化的蛋白磷酸酶, 钙调素结合蛋白

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规格/货号	是否有货	价格
100 units	请联系客服了解存货情况	$970.00

关于此项目

化学文摘社编号:

90371-51-0

UNSPSC Code:

51111800

NACRES:

NA.32

MDL number:

MFCD00130697

Specific activity:

≥2,500 units/mg protein

Biological source:

bovine

$970.00

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属性

biological source

bovine

Quality Segment

200

form

lyophilized powder

specific activity

≥2,500 units/mg protein

mol wt

dimer ~77 kDa, subunit mol wt 19-58 kDa

composition

Protein, 0.3-1.7% Lowry

solubility

H₂O: soluble

UniProt accession no.

P48452

storage temp.

−20°C

Gene Information

cow ... PPP3CA(286852)

说明

General description

Calcineurin (CaN) comprises CaN A and CaN B subunits. It exists as a heterodimer with a calmodulin-binding domain, catalytic site, a CaN B binding domain, and an autoinhibitory domain.

Application

Calcineurin from bovine brain has been used:

as a positive control in western blot analysis of oocytes and cumulus cells proteome
as a positive control in calmodulin (CaM)-agarose binding assay
to test its phosphatase activity in the presence of okadaic acid

Biochem/physiol Actions

Calcineurin (CaN) activity is stimulated by nickel (Nⁱ²⁺) and manganese (Mn²⁺) ions. It participates in the coupling of Ca²⁺signals. CaN may regulate oocyte growth and meiotic maturation in porcine. It also participates in gene regulation, cell survival, and death.

大脑中发现的主要钙调素结合蛋白。与 T 细胞活化和阿尔茨海默病中 τ 蛋白的过磷酸化有关的关键酶。

钙调磷酸酶是一种环孢菌素敏感、钙调节的丝氨酸-苏氨酸蛋白质磷酸酶，具有较广的底物特异性。它是大脑中发现的主要钙调素结合蛋白。最早鉴定为一种磷酸二酯酶 3′:5′ 环核苷酸 (PDE) 的钙调素活化抑制剂，钙调磷酸酶对腺苷酸环化酶具有类似的作用。是与 T 细胞活化相关的关键酶。也与阿尔茨海默病中 τ 蛋白的过磷酸化有关，并且已显示出可阻碍分化型 PC12 细胞中 τ 蛋白进行钙蛋白酶介导的蛋白质水解的特性。

Physical form

冻干粉，含 0.5% EGTA，缓冲盐及稳定剂。

Analysis Note

Sigma 在含 65mM KCl、8mM MgSO₄ 以及 0.3% 白蛋白且 pH 为 7.5 的 80mM Tris 中测试了其活性。

Other Notes

当与两单位活化剂 (P 2277) 以及 0.1mM Ca²⁺ 置于酶偶联系统中分析时，在 pH 7.5 和 30°C 下，一单位本品可以对活化的磷酸二酯酶 3′:5′-环核苷酸 (P 9529) 造成 50% 的抑制。

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此商品	SAB4200816	539568	HPA008233
Sigma-Aldrich C1907 钙调磷酸酶来源于牛大脑	Sigma-Aldrich SAB4200816 Anti-Calcineurin (α-Subunit) antibody, Mouse monoclonal	Sigma-Aldrich 539568 PP2B, Human, Recombinant, E. coli	Sigma-Aldrich HPA008233 Anti-PPP3CB antibody produced in rabbit
description lyophilized powder, ≥2,500 units/mg protein	description clone CN-A1, purified from hybridoma cell culture	description -	description Prestige Antibodies^® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
specific activity ≥2,500 units/mg protein	specific activity -	specific activity ≥300,000 U/mg, ≥50,000 units/mL	specific activity -
biological source bovine	biological source mouse	biological source -	biological source rabbit
Gene Information cow ... PPP3CA(286852)	Gene Information bovine ... PPP3CA(286852) human ... CALNA(5530) rat ... Caln1(363909)	Gene Information -	Gene Information human ... PPP3CB(5532)
form lyophilized powder	form -	form liquid	form buffered aqueous glycerol solution
mol wt dimer ~77 kDa, subunit mol wt 19-58 kDa	mol wt -	mol wt -	mol wt -
UniProt accession no. P48452	UniProt accession no. Q08209	UniProt accession no. -	UniProt accession no. P16298

存储类别

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

历史批次信息供参考:

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钙调磷酸酶来源于牛大脑

lyophilized powder, ≥2,500 units/mg protein