CDP-RO

CDP-Star^®

Disodium 2-chloro-5-(4-methoxyspiro {1,2-dioxetane-3,2′-(5′-chloro)tricyclo[3.3.1.1^3,7 ]decan}-4-yl)-1-phenyl phosphate

• Synonym(s): CDP-Star^® Chemiluminescent Substrate, ready-to-use, cdp-star, Disodium 2-chloro-5-(4-methoxyspiro[1,2-dioxetane-3,2′-(5-chlorotricyclo[3.3.1.1^3.7]decan])-4-yl]-1-phenyl phosphate
• Empirical Formula (Hill Notation): C₁₈H₁₉Cl₂O₇Na₂P
• CAS Number: 160081-62-9
• Molecular Weight: 495.20
• UNSPSC Code: 12352204
• PubChem Substance ID: 329777462
• MDL number: MFCD02094611
• NACRES: NA.54

Properties

• assay: >98%
• form: solution
• packaging: bottle of 1 mL (11685627001[25 mM]), bottle of 2 × 1 mL (11759051001[25 mM])
• manufacturer/tradename: Roche
• shipped in: wet ice
• storage temp.: 2-8°C
• SMILES string: Cl[C@]1(C2)C[C@H]3C4(OOC4(C5=CC(OP([O-])([O-])=O)=C(Cl)C=C5)OC)[C@@H]2C[C@@H](C1)C3
• InChI: 1S/C18H21Cl2O7P.2Na/c1-24-18(14-6-13(2-3-15(14)19)25-28(21,22)23)17(26-27-18)11-4-10-5-12(17)9-16(20,7-10)8-11;;/h2-3,6,10-12H,4-5,7-9H2,1H3,(H2,21,22,23);;/q;2*+1/p-2/t10-,11-,12+,16-,17?,18?;;
• InChI key: UKWLRLAKGMZXJC-VCCHNUGISA-L

Description

General description

Contents
25 mM solution (12.38 mg/ml), 100x concentration

Application

Used for the nonradioactive detection of labeled nucleic acid probes on membrane blots:

• Southern blot[1]
• Northern blot[2]
• Dot blot
• Colony and plaque hybridization
• Gel shift assays

Features and Benefits

CDP-Star is a chemiluminescent substrate for alkaline phosphatase that enables fast, ultra-sensitive detection of biomolecules by producing extremely rapid visible light. Light emission is recorded on X-ray film or on luminescence imager systems.

• Save time with a fast, ultra-sensitive substrate.
• Easily strip and reprobe membranes.
• Take multiple exposures of up to two days for each experiment.
• Reuse the substrate by filtering and storing in sodium azide.

Preparation Note

Storage conditions (working solution): Washing Buffer: 15 to 25 °C, stable
Maleic Acid Buffer: 15 to 25 °C, stable
Detection Buffer: 15 to 25 °C, stable
Blocking Solution: always prepare freshly
Antibody Solution: 2 to 8 °C

Analysis Note

Purity: Purified by HPLC.

Other Notes

• First exposure to X-ray film should be 5-15 minutes. Examine film and then determine other exposure times based on initial result.
• No preincubation step required.
• Apply substrate quickly to avoid gray shadows from substrate drops and drying of membrane (leads to uneven, high background).
• Do not use plastic wrap to cover blot; use hybridization bags, acetate sheet protectors, or two sheets of transparent film.
• Exposure times are shorter than with CSPD.
• Do not use nitrocellulose membranes.
• Repeat exposures can be made up to two days after the addition of substrate.

For life science research only. Not for use in diagnostic procedures.

Legal Information

CDP-Star is a registered trademark of Tropix, Inc.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: No data available
• flash_point_c: No data available