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17153

LS Differential Agar

NutriSelect® Plus, suitable for microbiology

Synonym(s):

Lactobacillus Streptococcus Differential Agar

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About This Item

NACRES:
NA.85
UNSPSC Code:
41171606
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sterility

non-sterile

Quality Segment

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L , beef extract, 5 g/L , casein enzymic hydrolysate, 10 g/L , L-cysteine hydrochloride, 0.3 g/L , dextrose, 20 g/L , papaic digest of soybean meal, 5 g/L , sodium chloride, 5 g/L , yeast extract, 5 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

6.1±0.2 (25 °C)

application(s)

bioburden testing
environmental
food and beverages, microbiology

suitability

nonselective and differential for Lactobacillus spp., nonselective and differential for Streptococcus spp.

Application

For the maximum growth and differentiation of Lactobacilli and Streptococci on the basis of colonial morphology, T.T.C. reduction and casein reaction.

Preparation Note

Suspend 65.3 g in 1 litre distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C and add the following sterile solutions previously kept warm at 50°C just prior to use; (1) 100 ml of 10% (w/v) aqueous solution of antibiotic free skim milk powder (Cat. No. No. 70166) sterilized at 121°C for 5 minutes.(2) 10 ml of TTC Solution (Cat. No. 17779). Mix well and pour into sterile petri plates.

Other Notes

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

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description

NutriSelect® Plus, suitable for microbiology

description

NutriSelect® Plus, powder, for bacteria, pack of 500 g, suitable for microbiology

description

NutriSelect® Plus, suitable for microbiology, according to ISO 11290-1:2017, according to ISO 11290-2:2017

description

NutriSelect® Basic, according to ISO 7899, powder, for Enterococcus spp., pack of 500 g, suitable for microbiology

form

powder

form

powder

form

powder

form

powder

suitability

nonselective and differential for Lactobacillus spp., nonselective and differential for Streptococcus spp.

suitability

selective and differential for coliforms, selective and differential for Lactobacillus spp., selective and differential for Proteus spp., selective and differential for Escherichia coli, bacteria (general purpose media)

suitability

selective and differential for Listeria spp.

suitability

Enterococcus spp., selective and differential for Streptococcus spp.

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

composition

papaic digest of soybean meal, 5 g/L , yeast extract, 5 g/L , sodium chloride, 5 g/L , beef extract, 5 g/L , agar, 15 g/L , casein enzymic hydrolysate, 10 g/L , L-cysteine hydrochloride, 0.3 g/L , dextrose, 20 g/L

composition

casein enzymic hydrolysate, 5 g/L , bromocresol green, 0.022 g/L , agar, 20 g/L , monopotassium phosphate, 0.55 g/L , dextrose, 50 g/L , manganese sulfate, 0.0025 g/L , actidione (cycloheximide), 0.004 g/L , magnesium sulfate, 0.125 g/L , yeast extract, 4 g/L , calcium chloride, 0.125 g/L , ferric chloride, 0.0025 g/L , potassium chloride, 0.425 g/L

composition

meat peptone, 18.0 g/L , glucose, 2.0 g/L , chromogenic substrate, 0.05 g/L , yeast extract, 10.0 g/L , agar, 15.0 g/L , sodium chloride, 5.0 g/L , casein enzymic hydrolysate, 6.0 g/L , disodium hydrogen phosphate anhydrous, 2.5 g/L , sodium pyruvate, 2.0 g/L , magnesium glycerophosphate, 1.0 g/L , magnesium sulfate, 0.5 g/L , lithium chloride, 10.0 g/L

composition

disodium hydrogen phosphate monohydrate, 4 g/L , sodium azide, 0.4 g/L , agar, 10 g/L , D(+)-glucose, 2 g/L , yeast extract, 5 g/L , tryptose, 20 g/L , 2,3,5-triphenyltetrazolium chloride, 0.1 g/L

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label


Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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