Key Documents

Orange G

Name: Orange G
Brand: SIGMA

Dye content, ≥80%, certified by the Biological Stain Commission, powder

Synonym(s):

1-Phenylazo-2-naphthol-6,8-disulfonic acid disodium salt, 7-Hydroxy-8-phenylazo-1,3-naphthalenedisulfonic acid disodium salt, Acid Orange 10, Wool Orange 2G

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About This Item

Empirical Formula (Hill Notation):

C₁₆H₁₀N₂Na₂O₇S₂

CAS Number:

1936-15-8

Molecular Weight:

452.37

UNSPSC Code:

12171500

NACRES:

NA.47

PubChem Substance ID:

24897871

EC Number:

217-705-6

MDL number:

MFCD00012457

Beilstein/REAXYS Number:

4120705

Colour Index Number:

16230

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Product Name

Orange G, certified by the BSC

agency

certified by the BSC

Quality Level

200

form

powder

composition

Dye content, ≥80%

technique(s)

microbe id | staining: suitable

color

orange to very dark orange

solubility

water: 1 mg/mL, clear

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

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Related Categories

Clinical & Diagnostics

Dry Dyes & Certified Stains

Hematology Stains

Histology Stains

Tissue Diagnostics

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Show Differences

1 of 1

This Item	861286	O3756	195235
Sigma-Aldrich O7252 Orange G	Sigma-Aldrich 861286 Orange G	Sigma-Aldrich O3756 Orange G	Sigma-Aldrich 195235 Orange II sodium salt
technique(s) microbe id \| staining: suitable	technique(s) microbe id \| staining: suitable	technique(s) nucleic acid detection: suitable	technique(s) -
color orange to very dark orange	color orange to very dark orange, and Red to Very Dark Red and Orange-Red and Red-Orange and Dark Red-Orange	color -	color -
application(s) diagnostic assay manufacturing hematology histology	application(s) diagnostic assay manufacturing hematology histology	application(s) -	application(s) diagnostic assay manufacturing hematology histology
form powder	form powder	form powder	form powder
solubility water: 1 mg/mL, clear	solubility H₂O: 1 mg/mL	solubility water: 1 mg/mL, clear, orange to red	solubility -
composition Dye content, ≥80%	composition Dye content, 80%	composition -	composition Dye content, ≥85%

General description

Orange G (C.l. 16230) is the disodium salt of benzeneazo-2-naphthol-6,8-disulfonic acid, C₁₆H₁₀N₂O₇S₂Na₂. Orange G, also known as wool orange 2G, is a component in several industry applications including cytology, histology, biology, and industrial staining. It is a component of Mallory′s connective tissue stain.

Application

Certified for use in Mallory′s connective tissue stain; Wilson-Ezrin method for pituitary acidophils; Kreyberg′s method for keratin and mucus; as a counterstain following Heidenhain′s iron hematoxylin on paraffin sections; Flemming′s triple stain on paraffin sections of onion root.

Orange G is typically used as a background or cytoplasmic stain for various biological samples including a common application within clincial cytology within Papanicolaou varients.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Integrity of chromatin and replicating DNA in nuclei released from fission yeast by semi-automated grinding in liquid nitrogen.

Givens RM

BMC Research Notes, 4, 499-499 (2011)

Optimization of Orange G dye adsorption by activated carbon of Thespesia populnea pods using response surface methodology.

M Arulkumar et al.

Journal of hazardous materials, 186(1), 827-834 (2011-01-15)

Thespesia populnea is a large tree found in the tropical regions and coastal forests of India. Its pods were used as a raw material for the preparation of activated carbon. The prepared activated carbon was used for the adsorptive removal

Biosorption of an Azo Dye by Aspergillus niger and Trichoderma sp. Fungal Biomasses.

Arumugam Sivasamy et al.

Current microbiology, 62(2), 351-357 (2010-07-21)

Biosorption is an eco-friendly and cost-effective method for treating the dye house effluents. Aspergillus niger and Trichoderma sp. were cultivated in bulk and biomasses used as biosorbents for the biosorption of an azo dye Orange G. Batch biosorption studies were

Decolourization of the azo dye Orange G in aqueous solution via a heterogeneous Fenton-like reaction catalysed by goethite.

Honghai Wu et al.

Environmental technology, 33(13-15), 1545-1552 (2012-09-20)

Decolourization of the azo dye Orange G (OG) was investigated by using goethite/H2O2 as a heterogeneous Fenton-like reagent. Five principle operational parameters, namely pH, ion strength, concentrations ofgoethite (alpha-FeOOH) and hydrogen peroxide (H2O2), and reaction temperature, were taken into account

Photocatalytic degradation of three azo dyes using immobilized TiO2 nanoparticles on glass plates activated by UV light irradiation: influence of dye molecular structure.

A R Khataee et al.

Journal of hazardous materials, 168(1), 451-457 (2009-03-13)

In order to discuss the effect of chemical structure on photocatalysis efficiency, the photocatalytic degradation of three commercial textile dyes (C.I. Acid Orange 10 (AO10), C.I. Acid Orange 12 (AO12) and C.I. Acid Orange 8 (AO8)) with different structure and

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