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P1925

Protein A–Agarose macrobeads

aqueous suspension

Synonym(s):

Protein A resin

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56
MDL number:
Form:
aqueous suspension
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form

aqueous suspension

Quality Segment

extent of labeling

~6 mg per mL

technique(s)

antibody protein purification: suitable

matrix activation

cyanogen bromide

matrix spacer

1 atom

capacity

~6 mg/mL binding capacity (human IgG)

storage temp.

2-8°C

General description

Protein A is an immunoglobulin (Ig)-binding protein that might be suitable for low-pressure antibody isolation. It is used to purify large amounts of IgG and binds to the Fc part of the antibody at the CH2–CH3 interface. Protein A-agarose is used for affinity chromatography, antibody purification and characterization, and protein A, G and L resins.

Application

Protein A–Agarose macrobeads has been used in immunoprecipitation. It has been used to study the effects of protein A immunoadsorption in patients with chronic dilated cardiomyopathy as well as to study multiple sclerosis and gastric cancer.

Physical form

Suspension in 0.9% NaCl containing 15Mm Sodium Azide

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This Item
P2545A8312SAB4200745
description

aqueous suspension

description

saline suspension

description

aqueous suspension

description

clone SPA-27, purified from hybridoma cell culture

form

aqueous suspension

form

saline suspension

form

aqueous suspension

form

buffered aqueous solution

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

200

capacity

~6 mg/mL binding capacity (human IgG)

capacity

≥15 mg/mL binding capacity (human IgG)

capacity

≥8 mg/mL binding capacity (peroxidase Type VI)

capacity

-

extent of labeling

~6 mg per mL

extent of labeling

~2 mg per mL

extent of labeling

40-80 μmol per mL

extent of labeling

-

matrix activation

cyanogen bromide

matrix activation

cyanogen bromide

matrix activation

epichlorohydrin

matrix activation

-


Hazard Codes

Precautionary Statements

Hazard Classifications

Aquatic Chronic 3

Storage Class

10 - Combustible liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)



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