PP0100
Trypsin Profile IGD Kit
Synonym(s):
In gel digestion kit, Proteomics grade Trypsin, TPCK treated
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About This Item
NACRES:
NA.56
UNSPSC Code:
12352200
Pricing and availability is not currently available.
Quality Segment
usage
1 kit sufficient for ≤100 applications
technique(s)
protein mass spectrometry: suitable
storage temp.
2-8°C
General description
The Trypsin Profile IGD Kit enables fast, efficient, and complete in-gel tryptic digestion of up to 100 excised protein spots. Digested proteins are ready for MALDI-MS (matrix-assisted laser desorption/ionization-mass spectrometry) and require no additional preparation. Because the Trypsin Profile IGD Kit contains Proteomics Grade Trypsin, a higher sequence coverage and fewer ambiguous autolytic peaks are observed in MALDI spectra.
Trypsin has been chemically modified through reductive methylation of the ε-amino groups of lysine to reduce autolysis and minimize autolytic fragments. In addition, it has been TPCK (tosyl phenylalanyl chloromethyl ketone) treated to remove residual chymotrypsin activity and then further purified by affinity chromatography, yielding a highly purified trypsin suitable for proteomics work.
Trypsin has been chemically modified through reductive methylation of the ε-amino groups of lysine to reduce autolysis and minimize autolytic fragments. In addition, it has been TPCK (tosyl phenylalanyl chloromethyl ketone) treated to remove residual chymotrypsin activity and then further purified by affinity chromatography, yielding a highly purified trypsin suitable for proteomics work.
Application
Features and Benefits
- Faster destaining than with alternative in-gel digest kits
- Includes 100% of the reagents needed to destain, digest, and extract proteins/peptides of interest
- Compatible with Coomassie, SYPRO™ Orange, SYPRO Ruby, and ProteoSilver™ Stained gels
- Resulting samples are ready for analysis by MALDI-MS or HPLC-MS
Legal Information
ProteoSilver is a trademark of Sigma-Aldrich Co. LLC
SYPRO is a trademark of Thermo-Fisher Scientific, Inc.
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This Item | |||
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| description - | description - | description - | description Dimethlyated, Proteomics Grade, recombinant, expressed in Pichia pastoris, ready-to-use solution |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| usage kit sufficient for ≤100 applications | usage - | usage - | usage - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Kit Components Only
Product No.
Description
- Destaining Solution Reconstituted to 75
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class
3 - Flammable liquids
Flash Point (°F)
35.6 °F - closed cup
Flash Point (°C)
2.0 °C - closed cup
WGK
WGK 2
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