PP0200
GlycoProfile™ I, In-Gel Deglycosylation Kit
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About This Item
UNSPSC Code:
12352200
EC Number:
232-615-7
NACRES:
NA.32
Pricing and availability is not currently available.
Application
The GlycoProfile™ Enzymatic In-gel N-Deglycosylation Kit is optimized to provide a convenient, reproducible method to N-deglycosylate and digest protein samples from one dimensional (1D) and two dimensional (2D) polyacrylamide gel pieces for subsequent mass spectrometry or HPLC analysis.
This procedure is suitable for Coomassie® Brilliant Blue and colloidal Coomassie stained gels. Silver stained gels may also be used if properly destained.
This procedure is suitable for Coomassie® Brilliant Blue and colloidal Coomassie stained gels. Silver stained gels may also be used if properly destained.
Biochem/physiol Actions
The kit includes Proteomics Grade PNGase F and trypsin enzymes necessary for N-linked deglycosylation and tryptic digestion, respectively. The samples can then be desalted and concentrated for analysis by MALDI-TOF MS or electrospray MS with subsequent database searching.
Preparation Note
Each kit contains reagents sufficient to deglycosylate and digest up to 10 samples.
Legal Information
GlycoProfile is a trademark of Sigma-Aldrich Co. LLC
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| description - | description sufficient for 20 samples | description Premixed solutions for casting bis-tris polyacrylamide gels; TMKIT-10 includes 36 mL resolving solution and 20 mL stacking solution; TMKIT-60 includes 216 mL resolving solution and 120 mL stacking solution | description - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shelf life ≥1 yr at 2‑8 °C | shelf life - | shelf life - | shelf life - |
Kit Components Only
Product No.
Description
- Acetonitrile, ≥99.93%, BioSyn® 50 mL
Kit Components Also Available Separately
Product No.
Description
SDS & Pricing
signalword
Danger
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
3 - Flammable liquids
flash_point_f
35.6 °F
flash_point_c
2.0 °C
wgk
WGK 3
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