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Substance P enhances the proliferation and migration potential of murine bone marrow-derived mesenchymal stem cell-like cell lines.

Substance P enhances the proliferation and migration potential of murine bone marrow-derived mesenchymal stem cell-like cell lines.

Experimental and therapeutic medicine (2015-03-18)

Maria Jose Dubon, Ki-Sook Park

ABSTRACT

Due to the therapeutic characteristics of bone marrow (BM)-derived mesenchymal stem cells (MSCs), clinical trials are testing the use of autologous or allogeneic MSCs for the treatment of several conditions. These therapies require large numbers of MSCs and numerous studies are attempting to find substances that could enhance the egression of endogenous MSCs from the BM into the periphery and increase their proliferation

MATERIALS

Product Number

Brand

Product Description

USP

Glacial acetic acid, United States Pharmacopeia (USP) Reference Standard

Sigma-Aldrich

DAPI, for nucleic acid staining

Sigma-Aldrich

Ethylenediaminetetraacetic acid solution, 0.02% in DPBS (0.5 mM), sterile-filtered, BioReagent, suitable for cell culture

Sigma-Aldrich

Ethylenediaminetetraacetic acid, BioUltra, ≥99.0% (KT)

Sigma-Aldrich

Ethylenediaminetetraacetic acid, 99.995% trace metals basis

Sigma-Aldrich

Ethylenediaminetetraacetic acid, ACS reagent, 99.4-100.6%, powder

Sigma-Aldrich

Ethylenediaminetetraacetic acid, ≥98.0% (KT)

Sigma-Aldrich

Ethylenediaminetetraacetic acid, BioUltra, anhydrous, ≥99% (titration)

Sigma-Aldrich

Ethylenediaminetetraacetic acid disodium salt solution, BioUltra, pH 8.0, ~0.5 M in H₂O

Sigma-Aldrich

Ethylenediaminetetraacetic acid, anhydrous, crystalline, BioReagent, suitable for cell culture

Sigma-Aldrich

Ethylenediaminetetraacetic acid, purified grade, ≥98.5%, powder

Sigma-Aldrich

Ethylenediaminetetraacetic acid, Vetec^™, reagent grade, 98%

Sigma-Aldrich

Substance P, Neuropeptide found in high concentrations in the gut.

Supelco

4-tert-Octylphenol monoethoxylate solution, 10 μg/mL in acetone, analytical standard

Sigma-Aldrich

Acetic acid, natural, ≥99.5%, FG

Sigma-Aldrich

Acetic acid-¹²C₂, 99.9 atom % ¹²C

Sigma-Aldrich

Acetic acid, glacial, ACS reagent, ≥99.7%

Sigma-Aldrich

Acetic acid, suitable for luminescence, BioUltra, ≥99.5% (GC)

Supelco

Acetic acid, analytical standard

Sigma-Aldrich

Acetic acid, glacial, ReagentPlus^®, ≥99%

Sigma-Aldrich

Acetic acid, glacial, puriss., meets analytical specification of Ph. Eur., BP, USP, 99.8-100.5%

Sigma-Aldrich

Acetic acid, glacial, puriss., 99-100%

Sigma-Aldrich

Acetic acid, glacial, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8%

Sigma-Aldrich

Acetic acid solution, suitable for HPLC

Sigma-Aldrich

Acetic acid, glacial, ≥99.99% trace metals basis

Sigma-Aldrich

Acetic acid, ≥99.5%, FCC, FG

Supelco

4-tert-Octylphenol monoethoxylate solution, 1 μg/mL in acetone, analytical standard