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  • Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Analytical chemistry (2015-03-17)
Evgeni Eltzov, Avital Cohen, Robert S Marks
ABSTRACT

Indoor air pollution became a recent concern found to be oftentimes worse than outdoor air quality. We developed a tool that is cheap and simple and enables continuous monitoring of air toxicity. It is a biosensor with both a nondisposable (monitor) and disposable (calcium alginate pads with immobilized bacteria) elements. Various parameters to enhance its signal have been tested (including the effect of the pad's orientation, it's exposure to either temperature or time with the air toxicant analyte, and various concentrations thereof). Lastly, the sensor has demonstrated its ability to sense the presence of chemicals in a real, indoor environment. This is the first step in the creation of a sensitive and simple operative tool that may be used in different indoor environments.

MATERIALS
Product Number
Brand
Product Description

Supelco
Residual Solvent - Toluene, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Sodium chloride, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Chloroform, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
1-Octanol, ACS reagent, ≥99%
Sigma-Aldrich
Diethyl ether, puriss., contains ~5 mg/L 2,6-di-tert.-butyl-4-methylphenol as stabilizer, meets analytical specification of Ph. Eur., BP, ≥99.5% (GC)
Sigma-Aldrich
Acetone, histological grade, ≥99.5%
Sigma-Aldrich
N,N-Dimethylformamide, biotech. grade, ≥99.9%
Sigma-Aldrich
Chloroform, ACS spectrophotometric grade, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
Sigma-Aldrich
Chloroform, puriss. p.a., reag. ISO, reag. Ph. Eur., 99.0-99.4% (GC)
Sigma-Aldrich
Acetone, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.5% (GC)
Sigma-Aldrich
Piperidine, biotech. grade, ≥99.5%
Sigma-Aldrich
Diethyl ether, ACS reagent, anhydrous, ≥99.0%, contains BHT as inhibitor
Sigma-Aldrich
Methanol, puriss., meets analytical specification of Ph Eur, ≥99.7% (GC)
Sigma-Aldrich
Diethyl ether, anhydrous, ACS reagent, ≥99.0%, contains BHT as inhibitor
Sigma-Aldrich
Tetrahydrofuran, ACS reagent, ≥99.0%, contains 200-400 ppm BHT as inhibitor
Supelco
Acetone, Pharmaceutical Secondary Standard; Certified Reference Material
USP
Acetone, United States Pharmacopeia (USP) Reference Standard
Supelco
2-Mercaptoethanol, derivatization grade (HPLC), LiChropur, ≥99.0% (GC)
Sigma-Aldrich
Chloroform, ReagentPlus®, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
Sigma-Aldrich
Methanol, ACS reagent, ≥99.8%
Sigma-Aldrich
Diethyl ether, ACS reagent, ≥98.0%, contains ≤2% ethanol and ≤10ppm BHT as inhibitor
Sigma-Aldrich
Methanol, Absolute - Acetone free
SAFC
Formaldehyde solution, contains 10-15% methanol as stabilizer, 37 wt. % in H2O
Sigma-Aldrich
Acetone, ACS reagent, ≥99.5%
Sigma-Aldrich
Acetone, Laboratory Reagent, ≥99.5%
Sigma-Aldrich
N,N-Dimethylformamide, ACS reagent, ≥99.8%
Sigma-Aldrich
Chloroform, contains ethanol as stabilizer, ACS reagent, ≥99.8%
Sigma-Aldrich
Methanol, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8% (GC)
Sigma-Aldrich
Methanol, ACS reagent, ≥99.8%
Sigma-Aldrich
Tetrahydrofuran, contains 200-400 ppm BHT as inhibitor, ACS reagent, ≥99.0%