HPLC Analysis of Nitroaromatic Compounds Using Ascentis® Express Biphenyl
CONDITIONS
column
Ascentis Express Biphenyl, 10 cm x 2.1mm I.D., 2.7 μm particles (64065-U)
mobile phase
[A]:Water; [B]:Methanol; (50:50, A:B)
flow rate
0.5 mL/min
pressure
7150 psi (492 bar)
column temp.
35 °C
detector
UV, 254 nm
injection
1 μL
sample
Toluene, Nitrobenzene;1,3,5-Trinitrobenzene; 2,4-Dinitrotoluene. 100 μg/mL in 50:50 A:B
Description
Analysis Note
This application demonstrates the separation of selected nitroaromatic compound using Ascentis Express Biphenyl. The biphenyl stationary phase is well suited to these substituted aromatic compounds due to interactions between the aromatic stationary phase and the electropositive benzene ring of the analyte. Unique selectivity is observed running this reversed phase separation using methanol mobile phase conditions
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany