69890

MES hydrate

BioUltra, ≥99.5% (T)

• Synonym(s): 2-(N-Morpholino)ethanesulfonic acid hydrate, 4-Morpholineethanesulfonic acid
• สูตรเอมพิริกัล (Hill Notation): C₆H₁₃NO₄S · xH₂O
• CASE番号: 1266615-59-1
• น้ำหนักโมเลกุล: 195.24 (anhydrous basis)
• UNSPSC Code: 12161700
• eCl@ss: 32129211
• PubChem Substance ID: 57652220
• NACRES: NA.25
• MDL number: MFCD00149409

คุณสมบัติ

• product line: BioUltra
• Quality Segment: 200
• assay: ≥99.5% (T)
• form: powder or crystals
• impurities: insoluble matter, passes filter test
• ign. residue (900 °C): ≤0.05%
• pH: 2.5-4.0 (25 °C, 0.5 M in H₂O)
• useful pH range: 5.5-6.7
• pKa: 6.1
• solubility: H₂O: 0.5 M at 20 °C, clear, colorless
• anion traces: chloride (Cl^-): ≤50 mg/kg, sulfate (SO₄^2-): ≤100 mg/kg
• cation traces: Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤20 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg
• λ: 0.5 M in H₂O
• UV absorption: λ: 260 nm A_max: 0.025, λ: 280 nm A_max: 0.020
• SMILES string: O.OS(=O)(=O)CCN1CCOCC1
• InChI: 1S/C6H13NO4S.H2O/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);1H2
• InChI key: MIIIXQJBDGSIKL-UHFFFAOYSA-N

คำอธิบาย

General description

MES is one of the "Good" buffers developed for biological applications. MES is not recommended at pH 7.4; other buffers should be considered. All qualities are tested for trace ions and all can be used for biological and biochemistry applications. This product is used in biochemical and biological techniques where highly purified products are needed.

Preparation Note

A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa=±1). Alternatively, volumes of equimolar MES free acid and sodium MES can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published.

Other Notes

Solubility/Stability: MES is soluble in water, giving a clear colourless solution at concentrations of 0.5M or higher. The pH of a solution should be between 2.5 and 5, depending on the concentration. Solutions are stable at 2-8°C for months.
Sterilization: Sterilization should be by filteration through 0.2μM filters. Autoclaving is not recommended by any sulfonic acid buffers. If buffers must be nuclease-free, it is best to treat the water, then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably. The identity of the yellow breakdown product is unknown.

Biological buffer; useful pH-range 5.8-6.5; Mobile phase component in brain tubulin isolation by amino-activated gel chromatography; Buffer in plant tissue culture.

Easily compare specifications for MES hydrate products with the MES hydrate specification table.

ข้อมูลความปลอดภัย

• คลาสการจัดเก็บ: 13 - Non Combustible Solids
• มีอะไรเกิดขึ้น?: WGK 1
• จุดติดไฟ (°F): Not applicable
• จุดวาบไฟ (°C): Not applicable
• อุปกรณ์ป้องกันส่วนบุคคล (PPE): Eyeshields, Gloves