93363

Trizma^® hydrochloride

BioUltra, Molecular Biology, ≥99.0% (AT)

• Synonym(s): TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride
• สูตรเชิงเส้น: NH₂C(CH₂OH)₃ · HCl
• CASE番号: 1185-53-1
• น้ำหนักโมเลกุล: 157.60
• UNSPSC Code: 12161700
• NACRES: NA.25
• PubChem Substance ID: 57653541
• EC Number: 214-684-5
• Beilstein/REAXYS Number: 3675235
• MDL number: MFCD00012590

คุณสมบัติ

• grade: Molecular Biology
• Quality Segment: 200
• product line: BioUltra
• assay: ≥99.0% (AT)
• form: powder or crystals
• impurities: DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected
• ign. residue: ≤0.2% (as SO₄)
• loss: ≤0.2% loss on drying, 110 °C
• pH: 2.5-4.0 (25 °C, 4 M in H₂O)
• useful pH range: 7.0-9.0
• pKa (25 °C): 8.1
• mp: 150-152 °C
• solubility: H₂O: 4 M at 20 °C, clear, colorless
• anion traces: sulfate (SO₄^2-): ≤50 mg/kg
• cation traces: Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg
• λ: 4 M in H₂O
• UV absorption: λ: 260 nm A_max: 0.12, λ: 280 nm A_max: 0.08
• SMILES string: Cl.NC(CO)(CO)CO
• InChI: 1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
• InChI key: QKNYBSVHEMOAJP-UHFFFAOYSA-N

คำอธิบาย

Application

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma hydrochloride is used as a general reagent for the preparation of all types of Tris buffers.

Other Notes

Component of extraction buffer e.g. phenol extraction of DNA or RNA; Buffer component of separating and stacking gels in the characterization of in vitro translation products by SDS-PAGE.

Easily compare specifications for Trizma HCl products with the Trizma HCl specification table.

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

ข้อมูลความปลอดภัย

• คลาสการจัดเก็บ: 11 - Combustible Solids
• มีอะไรเกิดขึ้น?: WGK 1
• จุดติดไฟ (°F): Not applicable
• จุดวาบไฟ (°C): Not applicable
• อุปกรณ์ป้องกันส่วนบุคคล (PPE): dust mask type N95 (US), Eyeshields, Gloves