Minor adjustments were introduced to the procedure, specifically regarding the volumes of reagents used in certain steps. Nevertheless, the methodology remains essentially unchanged compared to the previous version. These modifications are of little significance and do not affect the duration or efficiency of the method, both of which remain equivalent to those previously established.
PLN70
GenElute™ Plasmid Miniprep Kit
sufficient for 70 purifications
คุณสมบัติด้านความยั่งยืน
Synonym(s):
GenElute™ Plasmid Kit, plasmid extraction kit, plasmid isolation kit, plasmid purification kit, Gen Elute
เข้าสู่ระบบ เพื่อดูราคาสำหรับองค์กรและสัญญา
เลือกขนาด
เปลี่ยนการดู
| ขนาด/SKU | ความพร้อมจำหน่าย | ราคา |
|---|
เกี่ยวกับสินค้านี้
NACRES:
NA.52
UNSPSC Code:
41105501
ราคาสินค้าและจำนวนสินค้าไม่สามารถดูได้ในขณะนี้
บริการทางเทคนิค
ต้องการความช่วยเหลือหรือไม่ ทีมนักวิทยาศาสตร์ที่มีประสบการณ์ของเราอยู่ที่นี่เพื่อคุณ
ให้เราช่วยเหลือusage
sufficient for 70 purifications
Quality Segment
sustainability
Sustainability features
technique(s)
DNA purification: suitable
test parameters
: <30 min kit run time, sample volume: 1-5 mL
storage temp.
15-25°C
General description
The GenElute Plasmid Miniprep Kit offers a simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures. By combining silica-binding technology and the convenience of a spin column format, up to 15 μg of high copy plasmid DNA can be recovered from 1-5 mL of E. coli culture in less than 30 minutes. Note that actual yield and optimal volume of culture to use depend on the plasmid and the culture medium.
An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto silica in the presence of high salts. Contaminants are then removed by a spin-wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer.
An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto silica in the presence of high salts. Contaminants are then removed by a spin-wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
Application
The DNA is ready for immediate use in applications such as restriction enzyme digestion, cloning, PCR, transformation, transcription, conventional, and automated sequencing.
Features and Benefits
- Purified plasmid DNA in less than 30 minutes
- Purify up to 15 μg of plasmid DNA
- No detectable genomic DNA or RNA contamination
- No phenol/chloroform extraction or alcohol precipitation required
Other Notes
For additional information, please see www.sigma-aldrich.com/genelutehp.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
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สินค้าชิ้นนี้ | |||
|---|---|---|---|
| description sufficient for 70 purifications | description sufficient for 70 preparations | description sufficient for 350 purifications | description sufficient for 350 preparations |
| technique(s) DNA purification: suitable | technique(s) DNA extraction: suitable | technique(s) DNA purification: suitable | technique(s) DNA extraction: suitable |
| usage sufficient for 70 purifications | usage sufficient for 70 preparations | usage sufficient for 350 purifications | usage sufficient for 350 preparations |
| greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics Designing Safer Chemicals |
| sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product |
| test parameters sample volume: 1-5 mL, : <30 min kit run time | test parameters - | test parameters sample volume: 1-5 mL bacterial culture, : <30 min kit run time | test parameters : <30 min kit run time, sample volume: 1-5 mL bacterial culture |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
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สำรวจสินค้าทั้งหมดของเราภายใต้ GenElute™ Plasmid Miniprep Kit
คำเตือน
Danger
รหัสอันตราย
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
อวัยวะเป้าหมาย
Central nervous system
คลาสการจัดเก็บ
3 - Flammable liquids
มีอะไรเกิดขึ้น?
WGK 2
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