This 10X solution contains Potassium Chloride, Potassium Phosphate, Sodium Chloride, and Sodium Phosphate Dibasic. Please see the link below to review the concentration of each component in this formulation:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/139/193/d1408for.pdf
D1408
Dulbecco’s Phosphate Buffered Saline
Liquid (10X), without MgCl₂ and CaCl₂, sterile-filtered, suitable for cell culture
Synonym(s):
DPBS
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About This Item
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Product Name
Dulbecco′s Phosphate Buffered Saline, 10×, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture
Quality Segment
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
sodium pyruvate: no
glucose: no (Dextro)
shipped in
ambient
storage temp.
room temp
Application
Dulbecco′s Phosphate Buffered Saline has been used:
- in microfluidic cell culture of mouse embryonic stem cells (mESC)
- to dissolve albumin for adsorption studies
- in PC12 cell culture experiments
Dulbecco′s Phosphate Buffered Saline 10X is a stock solution used to prepare 1X D-PBS in cell culture grade water (W3500). D-PBS is used to wash cells during preparation and serial transfer.
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This Item | |||
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| description 10×, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture | description With MgCl2 and CaCl2, liquid, sterile-filtered, suitable for cell culture | description With calcium chloride and magnesium chloride, 10×, liquid, sterile-filtered, suitable for cell culture | description Modified, without calcium chloride and magnesium chloride, powder, suitable for cell culture |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| Quality Level 400 | Quality Level 400 | Quality Level 400 | Quality Level 500 |
| form liquid | form liquid | form liquid | form powder |
| storage temp. room temp | storage temp. 2-8°C | storage temp. room temp | storage temp. 2-8°C |
| impurities endotoxin, tested | impurities endotoxin, tested | impurities - | impurities - |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility - |
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Storage Class
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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