F0500

Fast Blue RR Salt

crystalline

• Synonym(s): 4-Benzoylamino-2,5-dimethoxybenzenediazonium chloride hemi(zinc chloride) salt, Azoic Diazo No. 24
• Linear Formula: C₁₅H₁₄ClN₃O₃ · 1/2 ZnCl₂
• CAS Number: 14726-29-5
• Molecular Weight: 387.89
• NACRES: NA.47
• PubChem Substance ID: 24894722
• UNSPSC Code: 12171500
• EC Number: 238-777-5
• MDL number: MFCD00074762
• Colour Index Number: 37155

Properties

• Product Name: Fast Blue RR Salt, crystalline
• form: crystalline
• Quality Segment: 200
• technique(s): microbe id | staining: suitable
• solubility: water: 1 mg/mL, clear to hazy, yellow-green
• ε (extinction coefficient): ≥15000 at 326-332 nm, ≥7000 at 390-396 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: −20°C
• SMILES string: [Cl-].[Cl-].Cl[Zn]Cl.COc1cc([N+]#N)c(OC)cc1NC(=O)c2ccccc2.COc3cc([N+]#N)c(OC)cc3NC(=O)c4ccccc4
• InChI: 1S/2C15H13N3O3.4ClH.Zn/c2*1-20-13-9-12(18-16)14(21-2)8-11(13)17-15(19)10-6-4-3-5-7-10;;;;;/h2*3-9H,1-2H3;4*1H;/q;;;;;;+2/p-2
• InChI key: LLARVKAOQLUCQI-UHFFFAOYSA-L

Description

Application

Fast blue RR salt has been used in the immunohistochemistry to detect the activity of alkaline phosphatase. It has been used in the procedure of connective tissue staining using picrosirius red.

Biochem/physiol Actions

Fast blue RR is mainly used in histological stainings. It binds to the reaction product of alkaline phosphatase and precipitates it as an insoluble compound. The sites of alkaline phosphatase activity appears black. Fast blue RR is also used for the detection of esterase activity. It couples with naphthol (released from naphthol acetate) and forms an insoluble azo-dye which appears red/blue.

Safety Information

• Storage Class: 11 - Combustible Solids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, type N95 (US)
• Pictograms: GHS07
• Signal Word: Warning
• Hazard Codes: H319
• Precautionary Statements: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2