04-953

Anti-phospho-Smad2 (Ser465/467) Antibody, clone A5S, rabbit monoclonal

culture supernatant, clone A5S, Upstate^®

• Synonym(s): Anti-Anti-CHTD8, Anti-Anti-JV18, Anti-Anti-JV18-1, Anti-Anti-LDS6, Anti-Anti-MADH2, Anti-Anti-MADR2, Anti-Anti-hMAD-2, Anti-Anti-hSMAD2
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: A5S, monoclonal
• species reactivity: human, zebrafish, mouse, canine
• manufacturer/tradename: Upstate^®
• technique(s): western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_001003652.2; NM_005901.4
• UniProt accession no.: Q15796
• shipped in: dry ice
• target post-translational modification: phosphorylation (pSer465/pSer467)
• Gene Information: human ... SMAD2(4087)

Description

General description

55-60 kDa

SMAD2 or Mothers against decapentaplegic homolog 2 is a polypeptide that, as its name describes, is a homolog of the Drosophila gene: "Mothers against decepentaplegic". It belongs to the SMAD family of proteins, which belong to the TGFb superfamily of modulators. Like many other TGFb family members SMAD2 is involved in cell signalling. SMAD2 modulates signals of activin and TGFb′s. It interacts with SMAD anchor for receptor activation (SARA). The binding of ligands causes the phosphorylation of the SMAD2 protein and the dissociation from SARA and the association with SMAD4. It is subsequently transferred to the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD2 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.

Immunogen

KLH-conjugated, synthetic peptide corresponding to amino acids [pS]M[pS] surrounding sites Ser465/467 of human Smad2

Application

Detect phospho-Smad2 (Ser465/467) with Anti-phospho-Smad2 (Ser465/467) Antibody, clone A5S (Rabbit Monoclonal Antibody), that has been shown to work in WB.

Biochem/physiol Actions

Predicted to cross-react with X. laevis based on sequence homology.

Recognizes phosphorylated Smad2. No Cross-reactivity with Smad3.

Analysis Note

Control
HL60 cell lysate, HepG2 cells treated with TGFa, or HepG2 cells treated with TGFβ

Routinely evaluated by immunoblot analysis.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 05-953

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2